Effect of hyaluronic acid incorporation method on the stability and biological properties of polyurethane-hyaluronic acid biomaterials.
Effect of hyaluronic acid incorporation method on the stability and biological properties of polyurethane-hyaluronic acid biomaterials.
复制标题
DOI：
10.1007/s10856-013-5092-1
复制
发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Masters KS
中科院分区：
文献类型：
--
作者：
Ruiz A;Rathnam KR;Masters KS
小直径血管移植物的高失败率继续推动解决该临床问题的新材料和改性策略的开发，其中生物分子掺入通常通过各种生物材料的基于表面的改性来实现。在这项工作中，我们检查了生物分子掺入的方法（即，本体与表面改性）转化为聚氨酯（PU）聚合物，影响血管应用背景下的生物材料性能。具体而言，透明质酸（HA）通过本体共聚或共价表面束缚掺入到聚（醚氨基甲酸酯）中，并且所得PU-HA材料的特征在于物理和生物性质。通过表面或本体方法用HA改性PU产生的材料在静态条件下测试时，在抵抗蛋白质吸附、血小板粘附和细菌粘附的能力方面没有显着差异，同时支持内皮细胞培养。然而，只有批量改性的PU-HA材料能够在材料暴露于流动后完全保留这些特性，证明了保留掺入的HA并最小化酶降解、蛋白质吸附、血小板粘附和细菌粘附的上级能力。因此，尽管本体方法很少在生物分子附着的情况下实施，但这些结果证明了在本体而不是表面掺入HA时PU-HA的改进的性能。虽然仅在PU-HA的背景下进行了探索，但这些实验揭示的结果对血管移植物修饰策略的设计和评价具有更广泛的意义。
The high failure rate of small diameter vascular grafts continues to drive the development of new materials and modification strategies that address this clinical problem, with biomolecule incorporation typically achieved via surface-based modification of various biomaterials. In this work, we examined whether the method of biomolecule incorporation (i.e., bulk vs. surface modification) into a polyurethane (PU) polymer impacted biomaterial performance in the context of vascular applications. Specifically, hyaluronic acid (HA) was incorporated into a poly(ether urethane) via bulk copolymerization or covalent surface tethering, and the resulting PU-HA materials characterized with respect to both physical and biological properties. Modification of PU with HA by either surface or bulk methods yielded materials that, when tested under static conditions, possessed no significant differences in their ability to resist protein adsorption, platelet adhesion, and bacterial adhesion, while supporting endothelial cell culture. However, only bulk-modified PU-HA materials were able to fully retain these characteristics following material exposure to flow, demonstrating a superior ability to retain the incorporated HA and minimize enzymatic degradation, protein adsorption, platelet adhesion, and bacterial adhesion. Thus, despite bulk methods rarely being implemented in the context of biomolecule attachment, these results demonstrate improved performance of PU-HA upon bulk, rather than surface, incorporation of HA. Although explored only in the context of PU-HA, the findings revealed by these experiments have broader implications for the design and evaluation of vascular graft modification strategies.
登录
查看更多内容
影响因子：
3.6
作者：
AMIJI, M;PARK, K
通讯作者：
PARK, K
影响因子：
14
作者：
Amarnath, LP;Srinivas, A;Ramamurthi, A
通讯作者：
Ramamurthi, A
DOI：
10.1111/j.1749-6632.1992.tb42589.x
发表时间：
1992-10-13
期刊：
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES
影响因子：
--
作者：
HUBBELL, JA;MASSIA, SP;DRUMHELLER, PD
通讯作者：
DRUMHELLER, PD
影响因子：
--
作者：
Krijgsman, B;Seifalian, AM;Hamilton, G
通讯作者：
Hamilton, G
肝素改性 ePTFE 血管移植物的血液和血管细胞相容性。
影响因子：
14
作者：
Hoshi, Ryan A.;Van Lith, Robert;Jen, Michele C.;Allen, Josephine B.;Lapidos, Karen A.;Ameer, Guillermo
通讯作者：
Ameer, Guillermo