Differentially expressed long noncoding RNAs in RAW264.7 macrophages during Brucella infection and functional analysis on the bacterial intracellular replication.
Differentially expressed long noncoding RNAs in RAW264.7 macrophages during Brucella infection and functional analysis on the bacterial intracellular replication.
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DOI：
10.1038/s41598-022-25932-6
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发表时间：
2022-12-09
影响因子：
4.6
通讯作者：
Yu, Shengqing
中科院分区：
文献类型：
--
作者：
Guan, Xiang;Hu, Hai;Tian, Minxing;Zhuang, Hongxu;Ding, Chan;Yu, Shengqing
长非编码RNA(Long Non Coding RNAs，LncRNAs)是一组没有蛋白质编码潜能的功能性RNA分子，在大多数生物过程中发挥着重要作用。到目前为止，对lncRNAs在RAW264.7细胞中的表达谱及其对布鲁氏菌复制的影响还知之甚少。在这项研究中，我们通过高通量转录组分析来研究与布鲁氏菌S2308感染相关的差异表达的lncRNAs。其中8个、6个、130个和94个细胞内的lncRNAs在感染后4、8、24和48h有差异表达。此外，通过寻找位于小鼠染色体DNA上lncRNA基因座上游和下游的蛋白质编码基因，预测了1918个编码蛋白质的基因可能成为差异表达的lncRNAs的顺式靶基因。基因本体论和京都百科全书的基因和基因组分析表明，大多数lncRNA靶基因与流产杆菌感染有关。从转录组数据中挑选了14个LncRNA进行qRT-PCR验证，证实有13个差异表达。动物实验表明，通过qRT-PCR分析，有三个在体内存在差异表达。此外，通过抑制CRISPR/dCas9或锁定核酸转染法抑制LNC_000428的表达，在基因水平上下调Tnfrsf8的表达，增加布鲁氏菌的细胞内复制。因此，我们提供了布鲁氏菌感染诱导的lncRNAs对布鲁氏菌细胞内复制起作用的新证据。
Long noncoding RNAs (lncRNAs) are a group of functional RNA molecules without protein-coding potential and play vital roles in majority of biological processes. To date, the expression profiles of lncRNAs and their influence on Brucella replication in RAW264.7 cells are poorly understood. In this study, we performed high-throughput transcriptome analysis to investigate the differentially expressed lncRNAs associated with Brucella abortus S2308 infection. Of these, 8, 6, 130 and 94 cellular lncRNAs were differentially expressed at 4, 8, 24 and 48 h post-infection, respectively. Moreover, 1918 protein-coding genes are predicted as potential cis target genes of differentially expressed lncRNAs by searching protein-coding genes located at upstream and downstream of lncRNA loci on the chromosome DNA of Mus musculus. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that majority of lncRNA target genes were associated with B. abortus infection. Fourteen lncRNAs from transcriptome data were selected for qRT-PCR verification, confirming 13 were differentially expressed. Animal experiments revealed three were differentially expressed in vivo by qRT-PCR analysis. Furthermore, knockdown of LNC_000428 by CRISPR/dCas9 inhibition or Locked Nucleic Acids transfection downregulated Tnfrsf8 expression at mRNA level and increased Brucella intracellular replication. Thus, we provide a novel evidence that lncRNAs induced by Brucella-infection function on Brucella intracellular replication.
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影响因子：
4.4
作者：
Hu, Hai;Tian, Mingxing;Yu, Shengqing
通讯作者：
Yu, Shengqing
影响因子：
3.4
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影响因子：
2.1
作者：
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通讯作者：
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影响因子：
11.4
作者：
Amodio N;Stamato MA;Juli G;Morelli E;Fulciniti M;Manzoni M;Taiana E;Agnelli L;Cantafio MEG;Romeo E;Raimondi L;Caracciolo D;Zuccalà V;Rossi M;Neri A;Munshi NC;Tagliaferri P;Tassone P
通讯作者：
Tassone P
影响因子：
8.8
作者：
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通讯作者：
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