Gelatin Methacryloyl-Riboflavin (GelMA-RF) Hydrogels for Bone Regeneration.
Gelatin Methacryloyl-Riboflavin (GelMA-RF) Hydrogels for Bone Regeneration.
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DOI：
10.3390/ijms22041635
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发表时间：
2021-02-06
影响因子：
5.6
通讯作者：
Mitani A
中科院分区：
文献类型：
--
作者：
Goto R;Nishida E;Kobayashi S;Aino M;Ohno T;Iwamura Y;Kikuchi T;Hayashi JI;Yamamoto G;Asakura M;Mitani A
甲基丙烯酰明胶（GelMA）是一种用途广泛的生物材料，已被用于各种生物医学领域。UV光通常用于光交联这种材料;然而，它的使用引起了一些生物安全问题。我们研究了可见波长（VW）光交联明胶基水凝胶的机械和生物学特性，以评估其作为骨破坏性疾病治疗中骨再生支架的可行性。加入Irgacure 2959或核黄素作为光引发剂以产生GelMA溶液。将GelMA溶液倒入模具中并暴露于UV或VW光。将载有KUSA-A1细胞的GelMA水凝胶交联，然后培养。力学特性表明，GelMA-RF水凝胶的刚度范围适合于成骨细胞分化。包封在GelMA水凝胶中的KUSA-A1细胞用VW光光聚合显示出比包封在水凝胶中的细胞用UV光光聚合显着更高的细胞活力。我们还表明，成骨细胞分化诱导条件下，成骨细胞分化的晚期阶段，在GelMA-RF水凝胶包封成骨细胞的成骨相关基因的表达显着增加。GelMA-RF水凝胶作为成骨细胞包封的优良支架。GelMA-RF水凝胶包封的成骨细胞不仅有可能帮助再生骨量，而且还可以治疗与骨破坏性疾病（如牙周炎）相关的复杂骨缺损。
Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been used in various biomedical fields. UV light is commonly used to photocrosslink such materials; however, its use has raised several biosafety concerns. We investigated the mechanical and biological properties of a visible-wavelength (VW)-light-crosslinked gelatin-based hydrogel to evaluate its viability as a scaffold for bone regeneration in bone-destructive disease treatment. Irgacure2959 or riboflavin was added as a photoinitiator to create GelMA solutions. GelMA solutions were poured into a mold and exposed to either UV or VW light. KUSA-A1 cell-laden GelMA hydrogels were crosslinked and then cultured. Mechanical characterization revealed that the stiffness range of GelMA–RF hydrogel was suitable for osteoblast differentiation. KUSA-A1 cells encapsulated in GelMA hydrogels photopolymerized with VW light displayed significantly higher cell viability than cells encapsulated in hydrogels photopolymerized with UV light. We also show that the expression of osteogenesis-related genes at a late stage of osteoblast differentiation in osteoblasts encapsulated in GelMA–RF hydrogel was markedly increased under osteoblast differentiation-inducing conditions. The GelMA–RF hydrogel served as an excellent scaffold for the encapsulation of osteoblasts. GelMA–RF hydrogel-encapsulated osteoblasts have the potential not only to help regenerate bone mass but also to treat complex bone defects associated with bone-destructive diseases such as periodontitis.
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影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
3.7
作者：
Eguchi T;Watanabe K;Hara ES;Ono M;Kuboki T;Calderwood SK
通讯作者：
Calderwood SK
影响因子：
14
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
6.6
作者：
Noshadi I;Hong S;Sullivan KE;Shirzaei Sani E;Portillo-Lara R;Tamayol A;Shin SR;Gao AE;Stoppel WL;Black LD III;Khademhosseini A;Annabi N
通讯作者：
Annabi N
影响因子：
4.8
作者：
Matsubara, Takuma;Kida, Kumiko;Yoneda, Toshiyuki
通讯作者：
Yoneda, Toshiyuki