Genomic analyses of heat stress transcription factors (HSFs) in simulated drought stress response and storage root deterioration after harvest in cassava

Genomic analyses of heat stress transcription factors (HSFs) in simulated drought stress response and storage root deterioration after harvest in cassava
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木薯收获后模拟干旱胁迫反应和贮藏根恶化中热胁迫转录因子(HSF)的基因组分析

DOI:
10.1007/s11033-020-05673-3
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发表时间:
2020-07
影响因子:
2.8
通讯作者:
Hu Wei
Hu Wei
中科院分区:
生物学4区
文献类型:
--
作者:
Zeng Jian;Wang Cheng;Wu Chunlai;Liao Fengfeng;Mo Jiajia;Ding Zehong;Tie Weiwei;Yan Yan;Hu Wei

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热休克因子(HSF)在各种植物胁迫反应中发挥着至关重要的作用。然而,目前对重要作物木薯中HSFs的了解仍然不足。在这项研究中,我们鉴定了 32 个木薯 HSF 基因(MeHSF),并根据系统发育分析和结构特征将它们分为三组(A、B、C)。保守基序分析表明 MeHSF 显示 HSF 转录因子特有的结构域。基因结构分析表明29MeHSF仅包含两个外显子。所有鉴定出的 32 个木薯MeHSF 分布在 13 条染色体上。其表达谱显示,不同的MeHSF在不同组织中表达差异,大多数高表达基因属于A组。相似的MeHSF在PEG和脱落酸(ABA)处理后上调,这表明这些MeHSF可能参与与ABA信号通路相关的模拟干旱胁迫的抵抗。此外,在木薯采后生理恶化(PPD)过程中诱导了几种MeHSF。我们的结果为未来 MeHSF 基因功能分析以提高木薯对非生物胁迫和 PPD 的耐受性提供了基础但重要的知识。
Heat shock factors (HSFs) play crucial roles in various plant stress responses. However, the current knowledge about HSFs in cassava, an important crop, is still insufficient. In this research, we identified 32 cassavaHSFgenes (MeHSFs) and clustered them into three groups (A, B, C) based on phylogenetic analysis and structural characteristics. Conserved motif analyses showed that MeHSFs display domains characteristic to HSF transcription factors. Gene structure analyses suggested that 29MeHSFscontained only two exons. All identified 32 cassavaMeHSFswere distributed on 13 chromosomes. Their expression profiles revealed that the differentMeHSFswere expressed differentially in different tissues, most high expression genes belonged to group A. The similarMeHSFswere up-regulated after treatment with both PEG and abscisic acid (ABA), which implied that theseMeHSFsmay participate in resistance to simulated drought stress associated with the ABA signaling pathway. In addition, severalMeHSFswere induced during postharvest physiological deterioration (PPD) in cassava. Our results provided basic but important knowledge for future gene function analysis ofMeHSFstoward efforts in improving tolerance to abiotic stress and PPD in cassava.
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