miR-129-5p inhibits clear cell renal cell carcinoma cell proliferation, migration and invasion by targeting SPN.
miR-129-5p inhibits clear cell renal cell carcinoma cell proliferation, migration and invasion by targeting SPN.
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DOI：
10.1186/s12935-021-01820-3
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发表时间：
2021-05-17
影响因子：
5.8
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Gao B;Wang L;Zhang N;Han M;Zhang Y;Liu H;Sun D;Xiao X;Liu Y
我们的研究旨在探讨miR-129-5p/SPN轴在透明细胞肾细胞癌（ccRCC）中的作用机制，为ccRCC的靶向治疗提供新的方向。采用生物信息学方法从TCGA数据库中寻找与ccRCC相关的差异表达基因（DEGs）。采用qRT-PCR检测miR-129-5p和SPN mRNA表达，采用western bot检测SPN蛋白表达。通过生物信息学分析预测miR-129-5p在spn3 ' utr上的结合位点，并通过双荧光素酶测定验证其结合关系。采用CCK-8法、菌落形成法、创面愈合法和Transwell法测定ccRCC细胞增殖能力、细胞形成能力、细胞迁移和侵袭能力。流式细胞术检测细胞周期和凋亡情况。miR-129-5p在ccRCC中表达水平显著下调，而SPN表达水平显著上调。过表达miR-129-5p抑制ccRCC细胞的增殖、侵袭和迁移能力，诱导细胞周期阻滞于G0/G1期，促进细胞凋亡。双荧光素酶测定证实miR-129-5p与SPN存在结合关系。此外，过表达miR-129-5p显著降低了SPN在癌细胞中的表达，削弱了SPN对细胞增殖、迁移、侵袭和细胞周期进程的促进作用，导致细胞凋亡活性增强。我们的研究证实了miR-129-5p/SPN轴在ccRCC中的调控作用，为精准治疗ccRCC患者提供了新的潜在靶点。
Our study aims to investigate the mechanism of the miR-129-5p/SPN axis in clear cell renal cell carcinoma (ccRCC), providing a novel direction for the targeted therapy of ccRCC. Bioinformatics methods were implemented to find the differentially expressed genes (DEGs) associated with ccRCC from TCGA database. qRT-PCR was performed to detect miR-129-5p and SPN mRNA expression, while western bot was carried out for the detection of protein expression of SPN. Bioinformatics analysis was used to predict the binding sites of miR-129-5p on SPN 3’UTR, while dual-luciferase assay was conducted to verify their binding relationship. CCK-8 assay, colony formation assay, wound healing assay and Transwell assay were employed to measure ccRCC cell proliferative ability, cell formation ability, cell migratory and invasive abilities. Flow cytometry was implemented to assess cell cycle and apoptosis. miR-129-5p exhibited a significantly down-regulated expression level in ccRCC, while SPN showed a remarkably up-regulated expression level. Overexpressed miR-129-5p inhibited ccRCC cell proliferative, invasive and migratory capacities while induced cell cycle arrest in G0/G1 phase and promoted cell apoptosis. Dual-luciferase assay confirmed that there was a binding relationship between miR-129-5p and SPN. Moreover, overexpressed miR-129-5p remarkably reduced SPN expression in cancer cells, weakened the promoting effect of SPN on cell proliferation, migration, invasion and cell cycle progress, and led to enhanced cell apoptotic activity. Our study proves the regulatory effect of the miR-129-5p/SPN axis in ccRCC, and provides a novel potential target for precise treatment of patients with ccRCC.
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影响因子：
2.9
作者：
Jiang L;Liu Y;Ma C;Li B
通讯作者：
Li B
DOI：
10.1007/s00432-009-0726-x
发表时间：
2010-06-01
影响因子：
3.6
作者：
Yi, Zhengjun;Fu, Yurong;Ma, Chuanxiang
通讯作者：
Ma, Chuanxiang
影响因子：
4
作者：
Gao, Ge;Xiu, Dianhui;Wang, Zhixin
通讯作者：
Wang, Zhixin
影响因子：
2
作者：
Cao, Jingyi;Wang, Qichao;Wang, Qian
通讯作者：
Wang, Qian
MiR-129-5p 通过靶向钙调蛋白依赖性蛋白激酶 IV (CAMK4) 抑制肝癌生长
影响因子：
9
作者：
Li, Zhengzhao;Lu, Junyu;Zhang, Jianfeng
通讯作者：
Zhang, Jianfeng