Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude.
Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude.
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DOI：
10.3389/fpls.2016.01689
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发表时间：
2016
影响因子：
5.6
通讯作者：
Molassiotis A
中科院分区：
文献类型：
--
作者：
Karagiannis E;Tanou G;Samiotaki M;Michailidis M;Diamantidis G;Minas IS;Molassiotis A
环境在果实生理学中的作用已经确定；然而，关于海拔对水果品质性状影响的知识仍然缺乏。在此，对采自低海拔（平均 71.5 m）或高海拔（平均 495 m）的 16 个果园收获的桃果实（cv. June Gold）的果皮组织质量特征进行了分析。数据表明，商业采收阶段的可溶性固形物浓度和果实硬度不受海拔的影响。在高海拔环境中生长的桃子在商业收获阶段的皮肤中表现出更高水平的色素沉着和特定的抗氧化相关活性。不同发育阶段和生长海拔的皮肤提取物对 DNA 链断裂表现出不同的抗氧化能力。使用蛋白质组学方法进一步研究了海拔对皮肤组织的影响。成熟果实的蛋白质表达分析显示 42 种蛋白质的表达发生改变，这些蛋白质主要涉及防御、初级代谢、目的地/储存和能量的代谢途径。大多数这些蛋白质在低海拔地区上调。高海拔环境增加了多种蛋白质的积累，包括伴侣ClpC、伴侣ClpB、丙酮酸脱氢酶E1、TCP结构域类转录因子和脂氧合酶。我们还讨论了受海拔影响的蛋白质变异，考虑到它们在桃子成熟过程中的潜在作用。这项研究首次描述了桃子皮肤蛋白质组的特征，有助于提高我们对桃子对海拔反应的理解。
The role of environment in fruit physiology has been established; however, knowledge regarding the effect of altitude in fruit quality traits is still lacking. Here, skin tissue quality characters were analyzed in peach fruit (cv. June Gold), harvested in 16 orchards located in low (71.5 m mean), or high (495 m mean) altitutes sites. Data indicated that soluble solids concentration and fruit firmness at commercial harvest stage were unaffected by alitute. Peach grown at high-altitude environment displayed higher levels of pigmentation and specific antioxidant-related activity in their skin at the commercial harvest stage. Skin extracts from distinct developmental stages and growing altitudes exhibited different antioxidant ability against DNA strand-scission. The effects of altitude on skin tissue were further studied using a proteomic approach. Protein expression analysis of the mature fruits depicted altered expression of 42 proteins that are mainly involved in the metabolic pathways of defense, primary metabolism, destination/storage and energy. The majority of these proteins were up-regulated at the low-altitude region. High-altitude environment increased the accumulation of several proteins, including chaperone ClpC, chaperone ClpB, pyruvate dehydrogenase E1, TCP domain class transcription factor, and lipoxygenase. We also discuss the altitude-affected protein variations, taking into account their potential role in peach ripening process. This study provides the first characterization of the peach skin proteome and helps to improve our understanding of peach's response to altitude.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
1.9
作者：
Cantin, Celia M.;Gogorcena, Yolanda;Angeles Moreno, Maria
通讯作者：
Angeles Moreno, Maria
影响因子：
5.6
作者：
Chan Z
通讯作者：
Chan Z
影响因子：
4.3
作者：
Gharechahi, Javad;Hajirezaei, Mohammad-Reza;Salekdeh, Ghasem Hosseini
通讯作者：
Salekdeh, Ghasem Hosseini
影响因子：
6.4
作者：
Hu, Hao;Liu, Yong;Wang, You-Nian
通讯作者：
Wang, You-Nian