The Snail family member Worniu is continuously required in neuroblasts to prevent Elav-induced premature differentiation.
The Snail family member Worniu is continuously required in neuroblasts to prevent Elav-induced premature differentiation.
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DOI：
10.1016/j.devcel.2012.09.007
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发表时间：
2012-10-16
影响因子：
11.8
通讯作者：
Doe, Chris Q.
中科院分区：
文献类型：
--
作者：
Lai, Sen-Lin;Miller, Michael R.;Robinson, Kristin J.;Doe, Chris Q.
蜗牛家族转录因子以调节上皮间质转化（EMT）而闻名。果蝇 Snail 家族成员 Worniu 在其整个生命周期中都在神经祖细胞（神经母细胞）中进行特异性转录，并且 Weariu 突变体表现出神经母细胞分层（EMT 的一种形式）方面的缺陷。然而，Worniu 在神经母细胞形成之外的作用尚不清楚。我们对 Weariu 突变幼虫神经母细胞进行 RNA 测序，观察到细胞周期转录本减少，神经分化转录本增加。与这些基因组数据一致，通过实时成像，weariu 突变神经母细胞显示出前期/中期转变的显着延迟，并且保守神经元分化剪接因子 Elav 水平增加。降低Elav水平显着抑制了weariu突变体表型。我们得出的结论是，神经母细胞持续需要 Worniu 通过促进细胞周期进程和抑制过早分化来维持自我更新。
Snail family transcription factors are best known for regulating epithelial-mesenchymal transition (EMT). The Drosophila Snail family member Worniu is specifically transcribed in neural progenitors (neuroblasts) throughout their lifespan, and worniu mutants show defects in neuroblast delamination (a form of EMT). However, the role of Worniu in neuroblasts beyond their formation is unknown. We performed RNA-seq on worniu mutant larval neuroblasts and observed reduced cell cycle transcripts and increased neural differentiation transcripts. Consistent with these genomic data, worniu mutant neuroblasts showed a striking delay in prophase/metaphase transition by live imaging, and increased levels of the conserved neuronal differentiation splicing factor Elav. Reducing Elav levels significantly suppressed the worniu mutant phenotype. We conclude that Worniu is continuously required in neuroblasts to maintain self-renewal by promoting cell cycle progression and inhibiting premature differentiation.
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影响因子：
4.6
作者：
Chabu, Chiswili;Doe, Chris Q.
通讯作者：
Doe, Chris Q.
ELAV是果蝇神经元特异性蛋白，可介导产生一种替代剪接的神经蛋白异构体
影响因子：
9.2
作者：
Koushika, SP;Lisbin, MJ;White, K
通讯作者：
White, K
影响因子：
10.5
作者：
Lisbin, MJ;Qiu, J;White, K
通讯作者：
White, K
影响因子：
11.4
作者：
Ashraf, SI;Hu, XD;Ip, YT
通讯作者：
Ip, YT
影响因子：
2.5
作者：
Berger, Christian;Renner, Simone;Technau, Gerhard M.
通讯作者：
Technau, Gerhard M.