Magnesium-free self-assembly of multi-layer DNA objects.
Magnesium-free self-assembly of multi-layer DNA objects.
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DOI：
10.1038/ncomms2095
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发表时间：
2012
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
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DNA 分子自组装提供了构建用户定义的纳米级物体的途径，但对溶液中镁的明显要求限制了可以实现此类物体的实际用途的条件范围。在这里，我们报告了在单价离子存在下组装模板化多层 DNA 物体的条件，表明一般的二价阳离子或特别的镁都不是成功组装此类物体的必要成分。物体中不与模板分子形成热稳定双螺旋 DNA 结构域 (Tm>45°C) 的 DNA 链的百分比与获得折叠物体的钠需求量相关。通过合理的设计选择最大限度地减少这种弱结合链的比例可以提高折叠的产量。结果支持这样的观点：基于 DNA 的纳米器件可以针对各种目标环境进行设计和生产。 DNA 自组装可以提供一系列纳米级结构，但使用镁进行组装被认为是必不可少的。 Martin 和 Dietz 报道了在存在单价离子（而不是镁）的情况下组装模板化多层 DNA 结构的条件。
Molecular self-assembly with DNA offers a route for building user-defined nanoscale objects, but an apparent requirement for magnesium in solution has limited the range of conditions for which practical utility of such objects may be achieved. Here we report conditions for assembling templated multi-layer DNA objects in the presence of monovalent ions, showing that neither divalent cations in general or magnesium in particular are essential ingredients for the successful assembly of such objects. The percentage of DNA strands in an object that do not form thermally stable double-helical DNA domains (Tm>45 °C) with the template molecule correlated with the sodium requirements for obtaining folded objects. Minimizing the fraction of such weakly binding strands by rational design choices enhanced the yield of folding. The results support the view that DNA-based nanodevices may be designed and produced for a variety of target environments. Self-assembly of DNA can provide access to a range of nanoscale structures, but assembly using magnesium has been considered essential. Martin and Dietz report conditions that allow the assembly of templated, multi-layer DNA structures in the presence of monovalent ions, rather than magnesium.
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影响因子：
15
作者：
Ke, Yonggang;Douglas, Shawn M.;Liu, Minghui;Sharma, Jaswinder;Cheng, Anchi;Leung, Albert;Liu, Yan;Shih, William M.;Yan, Hao
通讯作者：
Yan, Hao
影响因子：
14.8
作者：
Scheres, Sjors H. W.;Nunez-Ramirez, Rafael;Marabini, Roberto
通讯作者：
Marabini, Roberto
影响因子：
14.9
作者：
Douglas SM;Marblestone AH;Teerapittayanon S;Vazquez A;Church GM;Shih WM
通讯作者：
Shih WM
影响因子：
10.8
作者：
Ke, Yonggang;Sharma, Jaswinder;Yan, Hao
通讯作者：
Yan, Hao
影响因子：
11.4
作者：
DUCKETT, DR;MURCHIE, AIH;LILLEY, DMJ
通讯作者：
LILLEY, DMJ