Genome-wide identification, splicing, and expression analysis of the myosin gene family in maize (Zea mays).
Genome-wide identification, splicing, and expression analysis of the myosin gene family in maize (Zea mays).
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玉米 (Zea mays) 肌球蛋白基因家族的全基因组鉴定、剪接和表达分析
DOI：
10.1093/jxb/ert437
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发表时间：
2014-03
影响因子：
6.9
通讯作者：
Song R
中科院分区：
文献类型：
--
作者：
Wang G;Zhong M;Wang J;Zhang J;Tang Y;Wang G;Song R
基于肌动蛋白的肌球蛋白系统对于植物细胞内膜系统和运输网络的组织和动态是必不可少的。植物含有两个独特的肌球蛋白，第VIII类和第XI类，后者在结构和功能上与动物和真菌的第V类肌球蛋白相似。人们对草中的肌球蛋白知之甚少，尽管草中含有几种具有重要农学意义的谷类作物。在这里，我们从玉米(Zea Mays)基因组中鉴定了14个肌球蛋白基因。玉米肌球蛋白基因相对较大的大小是因为它们的内含子要长得多，内含子中含有丰富的转座元件。系统发育分析表明，玉米肌球蛋白基因可分为VIII类和XI类，分别有3个和11个成员。除了XI-F亚群外，其余的亚群至少在一个分析的谱系中重复，并且在拟南芥中发生的重复事件比在玉米中发生的更广泛。只有两对玉米肌球蛋白是从片段复制中产生的。表达分析表明，大多数玉米肌球蛋白基因是普遍表达的，但少数成员(XI-1、-6和-11)表现出花药特异的模式，许多基因经历了广泛的选择性剪接。我们还在O1基因座发现了一个短的转录本，它在概念上编码了一种无头肌球蛋白，最可能的功能是在转录水平上，而不是在翻译水平上通过显性-负机制。总之，这些数据为玉米肌球蛋白基因的进化和功能特征提供了重要的见解，可以转移到其他禾本科植物肌球蛋白同源基因的鉴定和应用中。
The actin-based myosin system is essential for the organization and dynamics of the endomembrane system and transport network in plant cells. Plants harbour two unique myosin groups, class VIII and class XI, and the latter is structurally and functionally analogous to the animal and fungal class V myosin. Little is known about myosins in grass, even though grass includes several agronomically important cereal crops. Here, we identified 14 myosin genes from the genome of maize (Zea mays). The relatively larger sizes of maize myosin genes are due to their much longer introns, which are abundant in transposable elements. Phylogenetic analysis indicated that maize myosin genes could be classified into class VIII and class XI, with three and 11 members, respectively. Apart from subgroup XI-F, the remaining subgroups were duplicated at least in one analysed lineage, and the duplication events occurred more extensively in Arabidopsis than in maize. Only two pairs of maize myosins were generated from segmental duplication. Expression analysis revealed that most maize myosin genes were expressed universally, whereas a few members (XI-1, -6, and -11) showed an anther-specific pattern, and many underwent extensive alternative splicing. We also found a short transcript at the O1 locus, which conceptually encoded a headless myosin that most likely functions at the transcriptional level rather than via a dominant-negative mechanism at the translational level. Together, these data provide significant insights into the evolutionary and functional characterization of maize myosin genes that could transfer to the identification and application of homologous myosins of other grasses.
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影响因子：
5.3
作者：
Ojangu EL;Tanner K;Pata P;Järve K;Holweg CL;Truve E;Paves H
通讯作者：
Paves H
影响因子：
2.7
作者：
Jiang, Shu-Ye;Cai, Minnie;Ramachandran, Srinivasan
通讯作者：
Ramachandran, Srinivasan
影响因子：
7.4
作者：
Lee, YRJ;Liu, B
通讯作者：
Liu, B
影响因子：
7.4
作者：
Peremyslov, Valera V.;Mockler, Todd C.;Dolja, Valerian V.
通讯作者：
Dolja, Valerian V.
影响因子：
7.4
作者：
Peremyslov, Valera V.;Prokhnevsky, Alexey I.;Dolja, Valerian V.
通讯作者：
Dolja, Valerian V.