Evaluating the transcriptional landscape and cell-cell communication networks in chronically irradiated parotid glands.
Evaluating the transcriptional landscape and cell-cell communication networks in chronically irradiated parotid glands.
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DOI：
10.1016/j.isci.2023.106660
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发表时间：
2023-05-19
期刊：
影响因子：
5.8
通讯作者：
Chibly, Alejandro M.
中科院分区：
文献类型：
--
作者：
Rheinheimer, Brenna A.;Pasquale, Mary C.;Limesand, Kirsten H.;Hoffman, Matthew P.;Chibly, Alejandro M.
了解辐射后导致慢性唾液功能减退的转录景观将有助于确定损伤机制和开发再生疗法。我们呈现了来自照射后10个月收集的对照和照射小鼠腮腺的scRNA-seq分析。我们确定了一个人口的分泌细胞定义的Etv1，这可能是一个腺泡细胞前体的特异性表达。腺泡和Etv 1+分泌分别表达Ntrk 2和Erbb3，而这些受体的配体在肌上皮和基质细胞中表达。此外，我们的数据表明，分泌细胞和CD4 + CD8 + T细胞是最转录影响在慢性损伤与辐射，表明主动免疫参与。最后，对细胞间通讯网络的评估预测，神经营养素、神经调节素、ECM和免疫信号在照射后失调，因此可能在缺乏修复中发挥作用。这个资源将有助于了解细胞特异性途径，可能有针对性地修复辐射腺体的慢性损伤。Etv 1定义了一个离散的分泌细胞簇，可能是腺泡前体Ntrk 2和Erbb 3是高度特异性的分泌细胞受体CD4 + CD8 + T细胞和腺泡细胞在IR后具有最大的转录变化，IR后分泌细胞中的Ntrk 2、Erbb 3、ECM和免疫信号传导发生改变。
Understanding the transcriptional landscape that results in chronic salivary hypofunction after irradiation will help identify injury mechanisms and develop regenerative therapies. We present scRNA-seq analysis from control and irradiated murine parotid glands collected 10 months after irradiation. We identify a population of secretory cells defined by specific expression of Etv1, which may be an acinar cell precursor. Acinar and Etv1+ secretory express Ntrk2 and Erbb3, respectively while the ligands for these receptors are expressed in myoepithelial and stromal cells. Furthermore, our data suggests that secretory cells and CD4+CD8+T-cells are the most transcriptionally affected during chronic injury with radiation, suggesting active immune involvement. Lastly, evaluation of cell-cell communication networks predicts that neurotrophin, neuregulin, ECM, and immune signaling are dysregulated after irradiation, and thus may play a role in the lack of repair. This resource will be helpful to understand cell-specific pathways that may be targeted to repair chronic damage in irradiated glands. Etv1 defines a discrete cluster of secretory cells that may be acinar precursors Ntrk2 and Erbb3 are highly specific secretory cell receptors CD4+CD8+T-cells and acinar cells have the greatest transcriptional changes after IR Ntrk2, Erbb3, ECM, and immune signaling are altered in secretory cells after IR Cell biology; Immunology; Transcriptomics
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影响因子：
14.9
作者：
The Gene Ontology Consortium
通讯作者：
The Gene Ontology Consortium
影响因子：
4.8
作者：
Henson, BS;Eisbruch, A;Ship, JA
通讯作者：
Ship, JA
DOI：
10.1016/j.ijrobp.2008.09.036
发表时间：
2009-02-01
影响因子：
7
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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