Electrochemically prepared poly(L-lysine) and 3-hydroxyphenylboronic acid composite as a conventional adhesion material for rice suspension cells
Electrochemically prepared poly(L-lysine) and 3-hydroxyphenylboronic acid composite as a conventional adhesion material for rice suspension cells
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电化学制备聚（L-赖氨酸）和3-羟基苯基硼酸复合物作为水稻悬浮细胞的常规粘附材料
DOI：
10.1016/j.elecom.2020.106737
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发表时间：
2020-06
影响因子：
5.4
通讯作者：
Weisong Pan
中科院分区：
文献类型：
--
作者：
Mei Zeng;Tiean Zhou;Zhaohong Su;Weisong Pan
采用循环伏安法在金电极上制备了聚(L-赖氨酸)-3-羟基苯基硼酸(3-PBA)复合材料，并用电化学和石英晶体微天平对其进行了表征，并将其用于促进植物细胞的黏附。3-PBA可以与细胞壁上的糖基化合物相互作用形成带负电荷的共价化合物，而PLL增加了带正电荷的位置的数量，从而增强了与带负电荷的细胞壁的静电相互作用，两者都促进了细胞的黏附。利用QCM和傅里叶变换红外光谱(FTIR)研究了PLL-聚(3-PBA)复合材料在金电极表面的修饰。此外，通过循环伏安、电化学阻抗谱、QCM和光学显微镜观察，证实了PLL和3-PBA对水稻悬浮细胞黏附的协同作用。最后，利用QCM实时监测了水稻细胞在盐胁迫条件下与PLL-Poly(3-PBA)/Au的黏附动态过程。在40 mM的氯化钠作用下，细胞先变软，然后变硬。这项工作为将植物细胞固定在传感器上提供了一种新的常规方法，对于在细胞水平上研究植物在各种胁迫下的结构、功能和行为具有重要意义。
Poly(L-lysine) (PLL)-3-hydroxyphenylboronic acid (3-PBA) composite was prepared onto an Au electrode via cyclic voltammetry (CV), which was characterized by electrochemical and quartz crystal microbalance (QCM) techniques, and used to promote adhesion of plant cells. 3-PBA can interact with glycosyl compounds on the cell wall to form negatively charged covalent compounds while PLL increases the number of positively charged sites, which enhances the electrostatic interactions with the negatively charged cell wall, both promoting cell adhesion. The PLL-poly(3-PBA) composite was successfully modified on the Au electrode surface, as demonstrated by QCM and Fourier transform infrared spectrophotometry (FTIR). Moreover, we confirmed the synergistic effect of PLL and 3-PBA to adhere rice suspension cells using CV, electrochemical impedance spectroscopy (EIS), QCM and optical microscope observations. Finally, the dynamic process of rice cells’ adhesion to PLL-poly(3-PBA)/Au followed by salt stress treatment of NaCl was monitored in real-time by QCM. The cells softened at first then hardened under the action of 40 mM NaCl. This work presents a novel and conventional way to immobilize plant cells onto sensors, and has importance in the study of the structure, function and behavior of plants under various stresses at the cellular level.
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影响因子：
14
作者：
Crompton, K. E.;Goud, J. D.;Forsythe, J. S.
通讯作者：
Forsythe, J. S.
影响因子：
9.4
作者：
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通讯作者：
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影响因子：
2.9
作者：
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影响因子：
4.5
作者：
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通讯作者：
Ying Ma;Xiurong Yang
影响因子：
4.5
作者：
P. N. Barlett;J. Cooper
通讯作者：
P. N. Barlett;J. Cooper