Integrated taxonomy: traditional approach and DNA barcoding for the identification of filarioid worms and related parasites (Nematoda).
Integrated taxonomy: traditional approach and DNA barcoding for the identification of filarioid worms and related parasites (Nematoda).
复制标题
综合分类学：传统方法和DNA条形码，用于鉴定丝虫类蠕虫和相关寄生虫（Nematoda）。
DOI：
10.1186/1742-9994-6-1
复制
发表时间：
2009-01-07
影响因子：
2.8
通讯作者：
Casiraghi M
中科院分区：
文献类型：
--
作者：
Ferri E;Barbuto M;Bain O;Galimberti A;Uni S;Guerrero R;Ferté H;Bandi C;Martin C;Casiraghi M
本文比较了传统形态学方法和DNA条形码技术在区分丝虫类线虫种（线虫纲、旋尾目）中的适用性和有效性。对这些寄生虫进行可靠和快速的分类鉴定是正确诊断重要和广泛的寄生虫病的基础。比较具有不同参数的DNA条形码的性能，测量形态学和分子鉴定方法之间的相关性强度。分子距离估计进行了两个不同的线粒体标记（coxI和12S rDNA）和不同的组合的数据处理进行了比较，以提供一个更强大的工具，易于识别的蠕虫。DNA条形码和形态学鉴定的丝虫显示出高度的一致性。尽管coxI和12S rDNA都可以达到高质量的性能，但只有coxI显示是可管理的。比对算法、空位处理和用于定义阈值的标准都被发现影响12S rDNA标记的DNA条形码的性能。使用coxI和确定的核苷酸差异水平来划分物种边界，DNA条形码也可以用来推断潜在的新物种。综合方法允许达到更高的辨别力。结果清楚地显示了基于DNA和形态学的鉴定在哪里是一致的，在哪里不是。在我们的工作中检查的几乎所有物种的DNA和形态鉴定之间的一致性是非常强的。我们建议DNA条形码作为一个可靠的，一致的，和民主的工具，在寄生线虫的常规鉴定物种歧视。
We compared here the suitability and efficacy of traditional morphological approach and DNA barcoding to distinguish filarioid nematodes species (Nematoda, Spirurida). A reliable and rapid taxonomic identification of these parasites is the basis for a correct diagnosis of important and widespread parasitic diseases. The performance of DNA barcoding with different parameters was compared measuring the strength of correlation between morphological and molecular identification approaches. Molecular distance estimation was performed with two different mitochondrial markers (coxI and 12S rDNA) and different combinations of data handling were compared in order to provide a stronger tool for easy identification of filarioid worms. DNA barcoding and morphology based identification of filarioid nematodes revealed high coherence. Despite both coxI and 12S rDNA allow to reach high-quality performances, only coxI revealed to be manageable. Both alignment algorithm, gaps treatment, and the criteria used to define the threshold value were found to affect the performance of DNA barcoding with 12S rDNA marker. Using coxI and a defined level of nucleotide divergence to delimit species boundaries, DNA barcoding can also be used to infer potential new species. An integrated approach allows to reach a higher discrimination power. The results clearly show where DNA-based and morphological identifications are consistent, and where they are not. The coherence between DNA-based and morphological identification for almost all the species examined in our work is very strong. We propose DNA barcoding as a reliable, consistent, and democratic tool for species discrimination in routine identification of parasitic nematodes.
登录
查看更多内容
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
9.8
作者：
Hebert PD;Stoeckle MY;Zemlak TS;Francis CM
通讯作者：
Francis CM
影响因子：
1.9
作者：
Agatsuma, T;Iwagami, M;Bain, O
通讯作者：
Bain, O
影响因子：
9.6
作者：
Besansky, NJ;Severson, DW;Ferdig, MT
通讯作者：
Ferdig, MT
影响因子：
4.1
作者：
Lefebure, T.;Douady, C. J.;Gibert, J.
通讯作者：
Gibert, J.