Brain Monoamine Dysfunction in Response to Predator Scent Stress Accompanies Stress-Susceptibility in Female Rats.
Brain Monoamine Dysfunction in Response to Predator Scent Stress Accompanies Stress-Susceptibility in Female Rats.
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DOI：
10.3390/biom13071055
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发表时间：
2023-06-29
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
创伤后应激障碍（PTSD）在女性中很常见；然而，有关创伤后应激障碍的临床前研究主要是在雄性动物中进行的。我们利用捕食者气味应激（PSS）啮齿动物创伤后应激障碍模型，试图确定易受应激影响的雌性大鼠与创伤后应激障碍相关的脑区（内侧前额叶皮层（mPFC）、伏隔核（NAc）、海马背侧（dHIPP）和腹侧（vHIPP））的单胺浓度是否发生了改变。对雌性 Sprague-Dawley 大鼠进行单次 10 分钟的 PSS 暴露，并在四周内对其持续性失神、恐惧和焦虑样行为进行测试。根据大鼠在蔗糖偏好任务中的蔗糖消耗量和在高架加迷宫开放臂中花费的时间，将大鼠表型为应激易感者。收集脑组织，并使用高效液相色谱法对去甲肾上腺素、多巴胺、血清素及其代谢物进行定量分析。雌性大鼠的应激易感性与前脑皮质多巴胺和血清素周转率的增加有关。应激易感性还与 NAc 中多巴胺转化率升高和 vHIPP 中去甲肾上腺素增加有关。我们的研究结果表明，单次压力暴露后的压力易感性与对雌性大鼠单胺功能的长期影响有关。这些数据表明，减少单胺转换的干预措施（如 MAOIs）可能对治疗女性创伤后应激障碍有效。
Post-traumatic stress disorder (PTSD) is prevalent in women; however, preclinical research on PTSD has predominantly been conducted in male animals. Using a predator scent stress (PSS) rodent model of PTSD, we sought to determine if stress-susceptible female rats show altered monoamine concentrations in brain regions associated with PTSD: the medial prefrontal cortex (mPFC), nucleus accumbens (NAc), and dorsal (dHIPP) and ventral (vHIPP) hippocampus. Female Sprague–Dawley rats were exposed to a single, 10-min PSS exposure and tested for persistent anhedonia, fear, and anxiety-like behavior over four weeks. Rats were phenotyped as stress-Susceptible based on sucrose consumption in the sucrose preference task and time spent in the open arms of the elevated plus maze. Brain tissue was collected, and norepinephrine, dopamine, serotonin, and their metabolites were quantified using high-performance liquid chromatography. Stress-susceptibility in female rats was associated with increased dopamine and serotonin turnover in the mPFC. Susceptibility was also associated with elevated dopamine turnover in the NAc and increased norepinephrine in the vHIPP. Our findings suggest that stress-susceptibility after a single stress exposure is associated with long-term effects on monoamine function in female rats. These data suggest interventions that decrease monoamine turnover, such as MAOIs, may be effective in the treatment of PTSD in women.
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影响因子：
6.9
作者：
Koenen KC;Ratanatharathorn A;Ng L;McLaughlin KA;Bromet EJ;Stein DJ;Karam EG;Meron Ruscio A;Benjet C;Scott K;Atwoli L;Petukhova M;Lim CCW;Aguilar-Gaxiola S;Al-Hamzawi A;Alonso J;Bunting B;Ciutan M;de Girolamo G;Degenhardt L;Gureje O;Haro JM;Huang Y;Kawakami N;Lee S;Navarro-Mateu F;Pennell BE;Piazza M;Sampson N;Ten Have M;Torres Y;Viana MC;Williams D;Xavier M;Kessler RC
通讯作者：
Kessler RC
影响因子：
25
作者：
Isingrini, Elsa;Perret, Lea;Giros, Bruno
通讯作者：
Giros, Bruno
影响因子：
--
作者：
KESSLER, RC;SONNEGA, A;NELSON, CB
通讯作者：
NELSON, CB
影响因子：
6.1
作者：
ARORA, RC;FICHTNER, CG;CRAYTON, JW
通讯作者：
CRAYTON, JW
影响因子：
4.3
作者：
Katzman, Martin A;Struzik, Lukasz;McBride, Joanna C
通讯作者：
McBride, Joanna C