The tandem repeat domain in the Listeria monocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of vasodilator-stimulated phosphoprotein and profilin.
The tandem repeat domain in the Listeria monocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of vasodilator-stimulated phosphoprotein and profilin.
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DOI：
10.1083/jcb.135.3.647
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发表时间：
1996-11
影响因子：
7.8
通讯作者：
Portnoy, DA
中科院分区：
文献类型：
--
作者：
Smith, GA;Theriot, JA;Portnoy, DA
ActA蛋白负责真核细胞胞浆中基于肌动蛋白的单核细胞增多性李斯特菌的运动。对我们改变富含脯氨酸重复序列(PRR；共识序列DFPPPPTDEEL)的突变体的分析表明，PRR的数量与运动速度之间存在线性关系，每个重复大约贡献2-3微米/分钟。缺乏所有功能性PRR的突变体(由缺失或点突变产生)的移动速度是野生型的30%。间接免疫荧光显示PRRs直接与血管扩张剂刺激的磷酸蛋白(VASP)结合，并使Profilin定位于细菌表面。在PRRs之间交错的长重复序列通过一种独立于PRRs、Vasp和Profilin的机制增加了基于肌动蛋白的运动发生的频率。最后，一个表达低水平ActA的突变体表现出一个阈值；有很低比例的移动细菌，但当运动发生时，它是在野生型速率。这些结果表明，ActA蛋白至少引导三个可分离的事件：(1)不依赖于重复区域的肌动蛋白聚合的启动；(2)依赖于长重复序列和ActA数量的运动的启动；(3)依赖于PrRs的运动速率。
The ActA protein is responsible for the actin-based movement of Listeria monocytogenes in the cytosol of eukaryotic cells. Analysis of mutants in which we varied the number of proline-rich repeats (PRR; consensus sequence DFPPPPTDEEL) revealed a linear relationship between the number of PRRs and the rate of movement, with each repeat contributing approximately 2-3 microns/min. Mutants lacking all functional PRRs (generated by deletion or point mutation) moved at rates 30% of wild-type. Indirect immunofluorescence indicated that the PRRs were directly responsible for binding of vasodilator-stimulated phosphoprotein (VASP) and for the localization of profilin at the bacterial surface. The long repeats, which are interdigitated between the PRRs, increased the frequency with which actin-based motility occurred by a mechanism independent of the PRRs, VASP, and profilin. Lastly, a mutant which expressed low levels of ActA exhibited a phenotype indicative of a threshold; there was a very low percentage of moving bacteria, but when movement did occur, it was at wild-type rates. These results indicate that the ActA protein directs at least three separable events: (1) initiation of actin polymerization that is independent of the repeat region; (2) initiation of movement dependent on the long repeats and the amount of ActA; and (3) movement rate dependent on the PRRs.
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基于肌动蛋白单核细胞增生李斯特菌的运动：肌动蛋白组装是由于细菌表面的固定细丝末端的局部维护而产生的。
影响因子：
7.8
作者：
MARCHAND, JB;MOREAU, P;PAOLETTI, A;COSSART, P;CARLIER, MF;PANTALONI, D
通讯作者：
PANTALONI, D
DOI：
10.1073/pnas.87.11.4179
发表时间：
1990-06-01
影响因子：
11.1
作者：
HEADLEY, VL;PAYNE, SM
通讯作者：
PAYNE, SM
影响因子：
3.6
作者：
KOCKS, C;MARCHAND, JB;COSSART, P
通讯作者：
COSSART, P
影响因子：
3.1
作者：
KUHN, M;PREVOST, MC;SANSONETTI, PJ
通讯作者：
SANSONETTI, PJ
影响因子：
7.5
作者：
COSSART, P
通讯作者：
COSSART, P