Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection.
Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection.
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DOI：
10.3390/nu8120806
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发表时间：
2016-12-12
期刊：
影响因子：
5.9
通讯作者：
Licciardi PV
中科院分区：
文献类型：
--
作者：
Hoe E;Nathanielsz J;Toh ZQ;Spry L;Marimla R;Balloch A;Mulholland K;Licciardi PV
维生素D诱导多种生物学效应，包括骨骼健康、钙稳态以及最近的免疫功能的重要功能。鉴于流行病学研究的数据表明维生素D缺乏与感染风险增加有关，维生素D在感染期间的作用特别令人感兴趣。维生素D具有多种免疫调节功能，尽管其在细菌感染中的作用尚不清楚。在这项研究中，我们研究了维生素D的活性代谢产物1，25（OH）2D 3对外周血单核细胞（PBMC）和纯化的免疫细胞亚群的影响，这些免疫细胞亚群是从健康成人中分离出来的，它们是用细菌配体热灭活肺炎球菌血清型19 F（HK 19 F）和脂多糖（LPS）刺激的。我们发现1，25（OH）2D 3显着降低了两种配体的促炎细胞因子TNF-α、IFN-γ和IL-1β以及趋化因子IL-8（3至53倍），而抗炎IL-10则增加（2倍，p = 0.016）在HK 19 F刺激的单核细胞中。与维生素D充足的成人（> 50 nmol/L）相比，维生素D不足的成人（<50 nmol/L）中HK 19 F特异性IFN-γ的水平显著更高（11.7倍，p = 0.038）。维生素D还将促炎/抗炎平衡向抗炎表型转移，并增加单核细胞上的CD 14表达（p = 0.008），以响应LPS而不是HK 19 F刺激。这些结果表明，1，25（OH）2D 3可能是炎症反应的重要调节剂，并支持进一步的体内和临床研究，以确认维生素D在这种情况下的潜在益处。
Vitamin D induces a diverse range of biological effects, including important functions in bone health, calcium homeostasis and, more recently, on immune function. The role of vitamin D during infection is of particular interest given data from epidemiological studies suggesting that vitamin D deficiency is associated with an increased risk of infection. Vitamin D has diverse immunomodulatory functions, although its role during bacterial infection remains unclear. In this study, we examined the effects of 1,25(OH)2D3, the active metabolite of vitamin D, on peripheral blood mononuclear cells (PBMCs) and purified immune cell subsets isolated from healthy adults following stimulation with the bacterial ligands heat-killed pneumococcal serotype 19F (HK19F) and lipopolysaccharide (LPS). We found that 1,25(OH)2D3 significantly reduced pro-inflammatory cytokines TNF-α, IFN-γ, and IL-1β as well as the chemokine IL-8 for both ligands (three- to 53-fold), while anti-inflammatory IL-10 was increased (two-fold, p = 0.016) in HK19F-stimulated monocytes. Levels of HK19F-specific IFN-γ were significantly higher (11.7-fold, p = 0.038) in vitamin D-insufficient adults (<50 nmol/L) compared to sufficient adults (>50 nmol/L). Vitamin D also shifted the pro-inflammatory/anti-inflammatory balance towards an anti-inflammatory phenotype and increased the CD14 expression on monocytes (p = 0.008) in response to LPS but not HK19F stimulation. These results suggest that 1,25(OH)2D3 may be an important regulator of the inflammatory response and supports further in vivo and clinical studies to confirm the potential benefits of vitamin D in this context.
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