The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior.
The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior.
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酸性可降解高分子生物材料的界面pH及其对成骨细胞行为的影响
DOI：
10.1038/s41598-017-06354-1
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发表时间：
2017-07-28
影响因子：
4.6
通讯作者：
Pan H
中科院分区：
文献类型：
--
作者：
Ruan C;Hu N;Ma Y;Li Y;Liu J;Zhang X;Pan H
为促进成骨细胞的生长，建立了弱碱性环境。不幸的是，这与可生物降解聚合物在骨再生中的应用不一致，因为降解产物通常是酸性的。本研究以聚(D，L-丙交酯)和哌嗪基聚氨酯尿素(P-PUU)作为酸性可降解材料的代表材料，研究了它们的界面pH值的变化，以及成骨细胞在界面pH可调的情况下的行为。这些结果表明，降解产物的释放导致了界面pH的迅速下降，而碱性链段的引入可以缓解这一现象。相反，当与成骨细胞共同培养时，界面pH的变化呈上升趋势，表明细胞可以通过分泌细胞代谢产物来构建微环境，以满足自身的生存。此外，成骨细胞在基质上的行为表明，PP单位最多的P-PUU更有利于细胞生长和细胞的成骨分化。这是由于P-PUU表面的亲水性和适中的N%，这是促进早期细胞反应的关键因素，以及界面pH有助于增强成骨分化的效果。
A weak alkaline environment is established to facilitate the growth of osteoblasts. Unfortunately, this is inconsistent with the application of biodegradable polymer in bone regeneration, as the degradation products are usually acidic. In this study, the variation of the interfacial pH of poly (D, L-lactide) and piperazine-based polyurethane ureas (P-PUUs), as the representations of acidic degradable materials, and the behavior of osteoblasts on these substrates with tunable interfacial pH were investigated in vitro. These results revealed that the release of degraded products caused a rapid decrease in the interfacial pH, and this could be relieved by the introduction of alkaline segments. On the contrary, when culturing with osteoblasts, the variation of the interfacial pH revealed an upward tendency, indicating that cell could construct the microenvironment by secreting cellular metabolites to satisfy its own survival. In addition, the behavior of osteoblasts on substrates exhibited that P-PUUs with the most PP units were better for cell growth and osteogenic differentiation of cells. This is due to the hydrophilic surface and the moderate N% in P-PUUs, key factors in the promotion of the early stages of cellular responses, and the interfacial pH contributing to the enhanced effect on osteogenic differentiation.
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影响因子：
4.1
作者：
Livoti, Christine M.;Morgan, Jeffrey R.
通讯作者：
Morgan, Jeffrey R.
影响因子：
16.1
作者：
Narayanan G;Vernekar VN;Kuyinu EL;Laurencin CT
通讯作者：
Laurencin CT
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影响因子：
6.2
作者：
Lin, Manping;Wang, Huaiyu;Luo, Yanfeng
通讯作者：
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影响因子：
14
作者：
Lu, Tao;Wen, Jin;Chu, Paul K.
通讯作者：
Chu, Paul K.
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影响因子：
4
作者：
Liu, W.;Wang, T.;Lu, W. W.
通讯作者：
Lu, W. W.