The common ancestral core of vertebrate and fungal telomerase RNAs.
The common ancestral core of vertebrate and fungal telomerase RNAs.
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DOI：
10.1093/nar/gks980
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发表时间：
2013-01-07
影响因子：
14.9
通讯作者：
Chen JJ
中科院分区：
文献类型：
--
作者：
Qi X;Li Y;Honda S;Hoffmann S;Marz M;Mosig A;Podlevsky JD;Stadler PF;Selker EU;Chen JJ
端粒酶是一种具有固有端粒酶RNA(TER)成分的核糖核蛋白。在酵母中，TERs非常大，在二级结构上与脊椎动物或纤毛虫TERs几乎没有相似之处。为了更好地了解真菌端粒酶的进化，我们从芽殖酵母的姊妹分支--芽殖酵母亚门和芽生酵母菌亚门中鉴定了74个TERs。我们最初使用一种新的基于深度测序的方法从粗糙脉孢菌中鉴定出TER，并通过计算搜索从现有的真菌基因组数据库中鉴定出同源的TER序列。值得注意的是，来自这些非酵母真菌的TERs与脊椎动物TERs有许多共同的属性。对Pezizmycotina TERs中高度保守区域的比较系统发育分析发现，脊椎动物TERs中有两个核心结构域在二级结构上与伪结和CR4/5几乎相同。然后，我们分析了在体外重组的粗糙裂殖酵母和裂殖酵母端粒酶，结果表明，这两个系统中的两个RNA核心区都可以作为两个单独的RNA片段重组反式活性。此外，引物延伸脉冲追逐分析证实，重组的粗糙奈瑟氏菌端粒酶以较高的处理能力合成TTAGGG重复序列，这是脊椎动物端粒酶的共同属性。总体而言，这项研究揭示了脊椎动物和真菌TERs共同的祖先核心，并为真菌TER结构和功能的分子进化提供了见解。
Telomerase is a ribonucleoprotein with an intrinsic telomerase RNA (TER) component. Within yeasts, TER is remarkably large and presents little similarity in secondary structure to vertebrate or ciliate TERs. To better understand the evolution of fungal telomerase, we identified 74 TERs from Pezizomycotina and Taphrinomycotina subphyla, sister clades to budding yeasts. We initially identified TER from Neurospora crassa using a novel deep-sequencing–based approach, and homologous TER sequences from available fungal genome databases by computational searches. Remarkably, TERs from these non-yeast fungi have many attributes in common with vertebrate TERs. Comparative phylogenetic analysis of highly conserved regions within Pezizomycotina TERs revealed two core domains nearly identical in secondary structure to the pseudoknot and CR4/5 within vertebrate TERs. We then analyzed N. crassa and Schizosaccharomyces pombe telomerase reconstituted in vitro, and showed that the two RNA core domains in both systems can reconstitute activity in trans as two separate RNA fragments. Furthermore, the primer-extension pulse-chase analysis affirmed that the reconstituted N. crassa telomerase synthesizes TTAGGG repeats with high processivity, a common attribute of vertebrate telomerase. Overall, this study reveals the common ancestral cores of vertebrate and fungal TERs, and provides insights into the molecular evolution of fungal TER structure and function.
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DOI：
10.1126/science.1170633
发表时间：
2009-11-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
de Lange T
通讯作者：
de Lange T
影响因子：
14.9
作者：
Brown Y;Abraham M;Pearl S;Kabaha MM;Elboher E;Tzfati Y
通讯作者：
Tzfati Y
影响因子：
56.9
作者：
COHN, M;BLACKBURN, EH
通讯作者：
BLACKBURN, EH
影响因子：
10.5
作者：
LINGNER, J;HENDRICK, LL;CECH, TR
通讯作者：
CECH, TR
影响因子：
14.9
作者：
Bhattacharyya, A;Blackburn, EH
通讯作者：
Blackburn, EH