Blood meal source and mixed blood-feeding influence gut bacterial community composition in Aedes aegypti.
Blood meal source and mixed blood-feeding influence gut bacterial community composition in Aedes aegypti.
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DOI：
10.1186/s13071-021-04579-8
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发表时间：
2021-01-28
影响因子：
3.2
通讯作者：
Cáceres CE
中科院分区：
文献类型：
--
作者：
Muturi EJ;Njoroge TM;Dunlap C;Cáceres CE
吸血昆虫的肠道中有一个细菌群落，它可以对生物和非生物因素做出显着的反应。确定这些微生物群落结构的关键因素具有重要的生态和流行病学意义。我们使用黄热病蚊子，埃及伊蚊，调查混合血餐对媒介蚊子肠道菌群的影响。成年雌性动物实验性地喂食来自鸡、兔或鸡和兔血液混合物的糖或血液，并使用16S rRNA基因扩增和MiSeq测序来表征其肠道微生物群。三种血粉处理的蚊子的肠道细菌群落分别聚类，表明宿主物种身份和混合吸血是蚊子肠道细菌群落组成的关键决定因素。混合血粉有协同效应的操作分类单位（OTU）的丰富度和香农多样性指数，这表明，混合血液喂养可以抵消某些宿主物种的血粉的营养赤字。在这项研究中观察到的微生物群落是不同的，从类似的饲料Ae。埃及人从我们以前的研究。这些研究结果表明，媒介宿主的取食偏好可以影响肠道微生物的组成和多样性，这可能会影响病原体的获取和传播。研究结果还表明，在实验室内不同的微环境条件下可能发挥重要作用，在构建独立饲养的蚊子种群的微生物群落。
The guts of blood-sucking insects host a community of bacteria that can shift dramatically in response to biotic and abiotic factors. Identifying the key factors structuring these microbial communities has important ecological and epidemiological implications. We used the yellow fever mosquito, Aedes aegypti, to investigate the impact of mixed blood meals on gut microbiota of vector mosquitoes. Adult females were experimentally fed on sugar or blood from chicken, rabbit or a mixture of chicken and rabbit blood, and their gut microbiota were characterized using 16S rRNA gene amplification and MiSeq sequencing. The gut bacterial communities of mosquitoes fed on the three blood meal treatments clustered separately, suggesting that host species identity and mixed blood-feeding are key determinants of gut bacterial community composition in mosquitoes. Mixed blood meal had a synergistic effect on both operational taxonomic unit (OTU) richness and the Shannon diversity index, suggesting that mixed blood-feeding can offset the nutritional deficit of blood meals from certain host species. The microbial communities observed in this study were distinct from those identified from similarly fed Ae. aegypti from our previous study. These findings demonstrate that vector host-feeding preferences can influence gut microbial composition and diversity, which could potentially impact pathogen acquisition and transmission by the vector. The results also demonstrate that different microenvironmental conditions within the laboratory may play an important role in structuring the microbial communities of independently reared mosquito colonies.
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影响因子：
3.7
作者：
Apte-Deshpande A;Paingankar M;Gokhale MD;Deobagkar DN
通讯作者：
Deobagkar DN
影响因子：
4.6
作者：
Bando, Hironori;Okado, Kiyoshi;Guelbeogo, Wamdaogo M.;Badolo, Athanase;Aonuma, Hiroka;Nelson, Bryce;Fukumoto, Shinya;Xuan, Xuenan;Sagnon, N'Fale;Kanuka, Hirotaka
通讯作者：
Kanuka, Hirotaka
DOI：
10.1073/pnas.95.10.5700
发表时间：
1998-05-12
影响因子：
11.1
作者：
Luckhart, S;Vodovotz, Y;Rosenberg, R
通讯作者：
Rosenberg, R
DOI：
10.1073/pnas.0233733100
发表时间：
2003-01-21
影响因子：
11.1
作者：
LoGiudice, K;Ostfeld, RS;Keesing, F
通讯作者：
Keesing, F
DOI：
10.1098/rspb.2007.0664
发表时间：
2007-09-07
影响因子：
4.7
作者：
Lembo, T.;Haydon, D. T.;Cleaveland, S.
通讯作者：
Cleaveland, S.