Phagocytic activity is impaired in type 2 diabetes mellitus and increases after metabolic improvement.
Phagocytic activity is impaired in type 2 diabetes mellitus and increases after metabolic improvement.
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DOI：
10.1371/journal.pone.0023366
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Simó R
中科院分区：
文献类型：
--
作者：
Lecube A;Pachón G;Petriz J;Hernández C;Simó R
1)评估2型糖尿病患者外周血单核细胞（PBMC）是否存在吞噬活性受损; 2）确定吞噬活性的最终受损是否与血糖控制相关，是否可以通过改善血糖水平来逆转。前瞻性招募21例2型糖尿病患者和21例健康志愿者进行病例对照研究。此外，HbA 1c高于8%的患者（n = 12）住院，以完成5天的血糖强化治疗。 通过使用我们实验室基于DNA/RNA活性染色开发的改良流式细胞术程序来评估吞噬活性，以区分红细胞和碎片。该方法简单、灵敏度高、重现性好，并利用了流式细胞术中广泛使用的经典方法。与非糖尿病受试者相比，2型糖尿病患者的活化巨噬细胞百分比较低（54.00±18.93 vs 68.53±12.77%; p = 0.006）。检测到吞噬活性与空腹血糖（r =-0.619，p = 0.004）和HbA 1c（r =-0.506，p = 0.019）之间存在显著负相关性。 此外，多元线性回归分析显示，空腹血糖或HbA 1c与吞噬活性独立相关。此外，在接受代谢优化的患者亚组中，观察到吞噬活性显著增加（p = 0.029）。 2型糖尿病患者的血糖控制与吞噬细胞活性有关。我们的研究结果表明，吞噬活性的改善可以增加代谢优化的有益效果。
1) To evaluate whether peripheral blood mononuclear cells (PBMCs) from type 2 diabetic patients present an impairment of phagocytic activity; 2) To determine whether the eventual impairment in phagocytic activity is related to glycemic control and can be reversed by improving blood glucose levels. 21 type 2 diabetic patients and 21 healthy volunteers were prospectively recruited for a case-control study. In addition, those patients in whom HbA1c was higher than 8% (n = 12) were hospitalized in order to complete a 5-day intensification treatment of blood glucose. Phagocytic activity was assessed by using a modified flow cytometry procedure developed in our laboratory based on DNA/RNA viable staining to discriminate erythrocytes and debris. This method is simple, highly sensitive and reproducible and it takes advantage of classic methods that are widely used in flow cytometry. Type 2 diabetic patients showed a lower percentage of activated macrophages in comparison with non-diabetic subjects (54.00±18.93 vs 68.53±12.77%; p = 0.006) Significant negative correlations between phagocytic activity and fasting glucose (r = −0.619, p = 0.004) and HbA1c (r = −0.506, p = 0.019) were detected. In addition, multiple linear regression analyses showed that either fasting plasma glucose or HbA1c were independently associated with phagocytic activity. Furthermore, in the subset of patients who underwent metabolic optimization a significant increase in phagocytic activity was observed (p = 0.029). Glycemic control is related to phagocytic activity in type 2 diabetes. Our results suggest that improvement in phagocytic activity can be added to the beneficial effects of metabolic optimization.
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影响因子：
16.2
作者：
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