Molecular weight and monosaccharide composition of Astragalus polysaccharides.
Molecular weight and monosaccharide composition of Astragalus polysaccharides.
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DOI：
10.3390/molecules13102408
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发表时间：
2008-10-01
期刊：
影响因子：
--
通讯作者：
Wang PP
中科院分区：
文献类型：
--
作者：
Xu DJ;Xia Q;Wang JJ;Wang PP
从黄芪水提物中分离得到两种多糖(APS-I和APS-II)，经乙醇沉淀、脱蛋白、离子交换层析和凝胶过滤等步骤得到纯化。采用高效液相色谱凝胶渗透色谱(HPLCGPC)测定其相对分子质量，用折射率检测器(RID)和NH2色谱柱分析其单糖组成。结果表明，APS-I由阿拉伯糖和葡萄糖组成，APS-II由鼠李糖、阿拉伯糖和葡萄糖组成，摩尔比分别为1：3.45和1：6.25：17.86。APS-I和APS-II的相对分子质量分别为1,699,100 Da和1,197,600 Da。
Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH2 column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.
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影响因子：
5.4
作者：
Zhang, Ying-Wen;Wu, Chao-Yan;Cheng, Hei-Tang
通讯作者：
Cheng, Hei-Tang
影响因子：
5.6
作者：
Li, Shigang;Zhang, Yongqi;Zhao, Jianxiong
通讯作者：
Zhao, Jianxiong
影响因子：
9.7
作者：
Cho, William C. S.;Leung, Kwok N.
通讯作者：
Leung, Kwok N.
DOI：
10.1016/j.bbrc.2004.06.065
发表时间：
2004-08-06
影响因子：
3.1
作者：
Shao, BM;Xu, W;Gao, XM
通讯作者：
Gao, XM
影响因子：
2.9
作者：
Masuko, T;Minami, A;Lee, YC
通讯作者：
Lee, YC