Commitment to expression of the metalloendopeptidases, collagenase and stromelysin: relationship of inducing events to changes in cytoskeletal architecture.
Commitment to expression of the metalloendopeptidases, collagenase and stromelysin: relationship of inducing events to changes in cytoskeletal architecture.
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致力于表达金属肽酶，胶原酶和Stromelysin的表达：诱导事件与细胞骨架结构变化的关系。
DOI：
10.1083/jcb.102.3.697
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发表时间：
1986-03
影响因子：
7.8
通讯作者：
AGGELER, J
中科院分区：
文献类型：
--
作者：
WERB, Z;HEMBRY, RM;MURPHY, G;AGGELER, J
改变兔滑膜成纤维细胞形态的试剂诱导金属内肽酶、胶原酶和基质溶解素的合成。我们研究了这些金属内肽酶表达的承诺的细胞骨架变化的关系。细胞松弛素B（CB）或12-O-十四烷酰佛波醇-13-乙酸酯处理的细胞变圆，只有失去应力纤维的细胞表达胶原酶和基质溶解素，如通过免疫荧光测定的。我们集中在CB的影响，因为它的快速可逆。当CB加入1-24 h，然后移除时，细胞在30-60 min内重新扩散。CB处理使细胞参与随后的胶原酶和基质溶解素合成的最短时间为3 h。用2 μ g/ml的CB处理3-24 h，或用不同浓度的CB（0-2 μ g/ml）处理24 h后，酶活性和酶原的生物合成在24 h时均表现出逐渐增加的趋势。即使用2微克/毫升CB处理仅3小时后，当通过免疫荧光监测细胞时，超过85%的细胞对胶原酶和溶基质素均呈阳性。相反，当检查胶原酶和溶基质素表达对CB诱导浓度的依赖性时，如通过免疫荧光测定的，胶原酶和溶基质素阳性的细胞数目存在剂量依赖性增加。因此，在低浓度的CB（小于0.5微克/毫升），观察到异质性群体响应。这些结果表明，成纤维细胞的金属蛋白酶的诱导的承诺是一个随机的过程中，第二个信号，与破坏的肌动蛋白细胞骨架可能是胶原酶和基质分解素基因表达的限速。
Agents that alter the morphology of rabbit synovial fibroblasts induce synthesis of the metalloendopeptidases, collagenase and stromelysin. We studied the relationship of cytoskeletal changes to the commitment to expression of these metalloendopeptidases. Cells treated with cytochalasin B (CB) or 12-O-tetradecanoylphorbol-13-acetate rounded, and only cells that had lost their stress fibers expressed collagenase and stromelysin, as determined by immunofluorescence. We concentrated on the effects of CB because of its rapid reversibility. When CB was added for 1-24 h, then removed, the cells respread within 30-60 min. The minimum period of CB treatment that committed cells to the subsequent synthesis of collagenase and stromelysin was 3 h. After initial treatment with 2 micrograms/ml CB for 3-24 h, or with various concentrations of CB (0-2 micrograms/ml) for 24 h, both enzyme activity and biosynthesis of the proenzymes showed a graded increase when measured at 24 h. Even after treatment with 2 micrograms/ml CB for only 3 h, greater than 85% of all cells were positive for both collagenase and stromelysin when cells were monitored by immunofluorescence. In contrast, when the dependence of collagenase and stromelysin expression on the inducing concentration of CB was examined, there was a dose- dependent increase in the number of cells positive for collagenase and stromelysin, as determined by immunofluorescence. Thus, at low concentrations of CB (less than 0.5 micrograms/ml), a heterogeneous population response was observed. These results suggest that the commitment of fibroblasts to induction of the metalloproteinases is a stochastic process in which a second signal that correlates with the disruption of the actin cytoskeleton may be rate-limiting for collagenase and stromelysin gene expression.
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影响因子：
7.8
作者：
HAEUPTLE, MT;SUARD, YLM;KRAEHENBUHL, JP
通讯作者：
KRAEHENBUHL, JP
影响因子：
2.9
作者：
SWALLA, BJ;SOLURSH, M
通讯作者：
SOLURSH, M
影响因子：
7.8
作者：
SCHLIWA, M;NAKAMURA, T;EUTENEUER, U
通讯作者：
EUTENEUER, U
影响因子：
2.5
作者：
TSUNEMATSU, Y
通讯作者：
TSUNEMATSU, Y
影响因子：
64.5
作者：
CERVERA, M;DREYFUSS, G;PENMAN, S
通讯作者：
PENMAN, S