N,Cl-Codoped Carbon Dots from Impatiens balsamina L. Stems and a Deep Eutectic Solvent and Their Applications for Gram-Positive Bacteria Identification, Antibacterial Activity, Cell Imaging, and ClO(-) Sensing.
N,Cl-Codoped Carbon Dots from Impatiens balsamina L. Stems and a Deep Eutectic Solvent and Their Applications for Gram-Positive Bacteria Identification, Antibacterial Activity, Cell Imaging, and ClO(-) Sensing.
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凤仙花茎中的 N,Cl 共掺杂碳点和低共熔溶剂及其在革兰氏阳性细菌鉴定、抗菌活性、细胞成像和 ClO 传感中的应用
DOI：
10.1021/acsomega.1c04078
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发表时间：
2021-11-02
期刊：
影响因子：
4.1
通讯作者：
Gao D
中科院分区：
文献类型：
--
作者：
Liu S;Quan T;Yang L;Deng L;Kang X;Gao M;Xia Z;Li X;Gao D
在本研究中，我们首先以凤仙花为原料合成了无金属掺杂的N，Cl碳量子点（N，Cl-CD）。茎作为绿色前体在深共熔溶剂（DES）中。通过透射电子显微镜（TEM）、X射线光电子能谱（XPS）、X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、荧光光谱（FL）和紫外光谱（UV）对所制备的N，Cl-环糊精进行了表征。除了具有碳量子点（CD）的光稳定性高、尺寸小、毒性低、水溶性好、生物相容性好等特点外，N，Cl-CD还通过DES掺杂N和Cl元素，对革兰氏阳性菌表现出良好的识别性和选择性。带正电荷的N，Cl-环糊精不仅可以通过选择性荧光成像区分革兰氏阳性菌，而且对革兰氏阳性菌具有抗菌作用。通过对N，Cl-CD处理前后细菌的电位、活性氧和形态学分析，探讨了N，Cl-CD对屎肠球菌、金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌、铜绿假单胞菌和沙门氏菌的抑菌机理。此外，N，Cl-CD在癌细胞和正常细胞中表现出低细胞毒性和良好的细胞成像能力。此外，它们可以用作检测ClO-的荧光传感器，检测范围为100 nM至40 μM，检测限（LOD）为30 nM。综上所述，制备的N，Cl-环糊精可作为环境友好的革兰氏阳性菌鉴定和抗菌剂。此外，它们的细胞成像和ClO-检测能力也非常出色。
In this study, we first synthesized metal-free N,Cl-doped carbon dots (N,Cl-CDs) using Impatiens balsamina L. stems as green precursors in a deep eutectic solvent (DES). The obtained N,Cl-CDs were characterized through transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, fluorescence (FL) spectroscopy, and ultraviolet (UV) spectroscopy. In addition to the common features of carbon dots (CDs), such as high light stability, small size, low toxicity, good aqueous solubility, and favorable biocompatibility, these N,Cl-CDs exhibited excellent recognition and selectivity for Gram-positive bacteria by doping with N and Cl elements using DES. The N,Cl-CDs with positive charge cannot only differentiate Gram-positive bacteria by selective fluorescence imaging but also have antibacterial effects on Gram-positive bacteria. Through potential, ROS, and morphological analyses of bacteria before and after treatment with N,Cl-CDs, the antibacterial mechanisms of bacteriostasis, Enterococcus faecium, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Salmonella were explored. In addition, N,Cl-CDs demonstrated low cytotoxicity and good cell imaging ability in cancer and normal cells. Moreover, they can be used as a fluorescence sensor for the detection of ClO– with a detection range from 100 nM to 40 μM and a limit of detection (LOD) of 30 nM. In summary, the prepared N,Cl-CDs could be applied as environmentally friendly Gram-positive bacterial identification and antibacterial agents. Additionally, their cell imaging and ClO– detection abilities were outstanding.
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