A two-component system regulates hemin acquisition in Porphyromonas gingivalis.
A two-component system regulates hemin acquisition in Porphyromonas gingivalis.
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DOI：
10.1371/journal.pone.0073351
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Duncan MJ
中科院分区：
文献类型：
--
作者：
Scott JC;Klein BA;Duran-Pinedo A;Hu L;Duncan MJ
牙龈卟啉单胞菌是一种与牙周感染相关的革兰氏阴性厌氧菌。该菌具有少量的双组分信号转导系统，通过比较W83菌株和ATCC 33277菌株的基因组序列，发现后者是组氨酸激酶(PGN0752)的突变株，而同源反应调节因子(PGN0753)保持不变。用含有W83(PG0719)的功能性组氨酸激酶的atcc 33277转接子进行了基于基因芯片的转录图谱和芯片序列分析。数据显示，这个信号转导系统的调节子包含与获得氯化血红素有关的基因，包括牙龈痛，至少有三个运输系统，以及自我调节。通过电泳迁移率改变分析，证实了反应调节器的直接调控。此外，该系统似乎被氯化血红素激活，该调节器既是激活剂又是抑制物。
Porphyromonas gingivalis is a Gram-negative oral anaerobe associated with infection of the periodontia. The organism has a small number of two-component signal transduction systems, and after comparing genome sequences of strains W83 and ATCC 33277 we discovered that the latter was mutant in histidine kinase (PGN_0752), while the cognate response regulator (PGN_0753) remained intact. Microarray-based transcriptional profiling and ChIP-seq assays were carried out with an ATCC 33277 transconjugant containing the functional histidine kinase from strain W83 (PG0719). The data showed that the regulon of this signal transduction system contained genes that were involved in hemin acquisition, including gingipains, at least three transport systems, as well as being self-regulated. Direct regulation by the response regulator was confirmed by electrophoretic mobility shift assays. In addition, the system appears to be activated by hemin and the regulator acts as both an activator and repressor.
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