Toxicity of Six Insecticides on Codling Moth (Lepidoptera: Tortricidae) and Effect on Expression of Detoxification Genes
Toxicity of Six Insecticides on Codling Moth (Lepidoptera: Tortricidae) and Effect on Expression of Detoxification Genes
复制标题
六种杀虫剂对苹果蠹蛾（鳞翅目：卷蛾科）的毒性及对解毒基因表达的影响
DOI：
10.1093/jee/tov297
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发表时间：
2015-10
影响因子：
2.2
通讯作者：
Barros-Parada Wilson
中科院分区：
文献类型：
--
作者：
Yang Xue-Qing;Wu Zheng-Wei;Zhang Ya-Lin;Barros-Parada Wilson
苹果蠹蛾Cydia pomonella（L.）是一种重要的世界性水果害虫，已经进化出对几乎所有类别的常规杀虫剂的高水平抗性。新烟碱类杀虫剂是一种新的低风险生物合理杀虫剂，一直是一种有效的控制方法。本研究测定了常规杀虫剂和低风险杀虫剂对苹果蠹蛾3个解毒基因转录本丰度的毒力和亚致死效应。对苹果蠹蛾实验室菌株的生物测定表明，与其他五种农药相比，啶虫脒对三龄幼虫的经口毒性最高。结果还表明，啶虫脒表现出长期的效果，即使在120小时后，对苹果蠹蛾喂养。实时定量聚合酶链反应显示，解毒基因CYP 9A 61，CpGST 1，CpCE-1的差异诱导或抑制溴氰菊酯，氯氰菊酯，灭多威，西维因，吡虫啉，根据杀虫剂的类型;相反，CYP 9A 61，CpGST 1，CpCE-1的表达没有显着差异后，观察到与对照相比，啶虫脒暴露。这些结果表明，低风险的生物合理杀虫剂啶虫脒是一种有效的杀虫剂，没有诱导解毒基因，可以整合到苹果蠹蛾的管理。
Abstract The codling moth, Cydia pomonella (L.), is a key worldwide fruit pest that has evolved high levels of resistance to almost all classes of conventional insecticides. Neonicotinoids, a new reduced-risk biorational insecticide class, have remained an effective control approach. In this study, the toxicity and sublethal effect of conventional and reduced-risk biorational insecticides on transcripts abundance of three detoxification genes in codling moth were determined. Bioassays on a codling moth laboratory strain suggested that acetamiprid had the highest oral toxicity against the third-instar larvae compared with the other five pesticides. Results also indicated that acetamiprid exhibits long-term efficacy against codling moth even at 120 h post feeding. Real-time quantitative polymerase chain reaction showed that the detoxification genes CYP9A61, CpGST1, and CpCE-1 were differentially induced or suppressed by deltamethrin, cypermethrin, methomyl, carbaryl, and imidacloprid, depending on the type of insecticides; in contrast, no significant difference in CYP9A61, CpGST1, and CpCE-1 expressions were observed after acetamiprid exposure, when compared with the control. These results suggest that the reduced-risk biorational insecticide acetamiprid is an effective insecticide with no induction of detoxification genes and can be integrated into the management of codling moth.
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影响因子：
2.2
作者：
D. L. Cox;A. Knight;D. Biddinger;J. Lasota;B. Pikounis;L. Hull;R. A. Dybas
通讯作者：
D. L. Cox;A. Knight;D. Biddinger;J. Lasota;B. Pikounis;L. Hull;R. A. Dybas
影响因子：
5.6
作者：
Yang, Xue Qing;Liu, Ji Yuan;Zhang, Ya Lin
通讯作者：
Zhang, Ya Lin
影响因子：
5.6
作者：
Yang X;Li X;Zhang Y
通讯作者：
Zhang Y
DOI：
10.1093/jee/tou001
发表时间：
2015-02
期刊：
--
影响因子：
--
作者：
M. Reyes;W. Barros-Parada;C. Ramírez;E. Fuentes‐Contreras
通讯作者：
M. Reyes;W. Barros-Parada;C. Ramírez;E. Fuentes‐Contreras
影响因子：
2.2
作者：
H. R. Moffitt;S. Drake;H. Toba;P. Hartsell
通讯作者：
H. R. Moffitt;S. Drake;H. Toba;P. Hartsell