Human immunology studies using organ donors: Impact of clinical variations on immune parameters in tissues and circulation.
Human immunology studies using organ donors: Impact of clinical variations on immune parameters in tissues and circulation.
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DOI：
10.1111/ajt.14434
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Farber DL
中科院分区：
文献类型：
--
作者：
Carpenter DJ;Granot T;Matsuoka N;Senda T;Kumar BV;Thome JJC;Gordon CL;Miron M;Weiner J;Connors T;Lerner H;Friedman A;Kato T;Griesemer AD;Farber DL
器官捐赠者是用于挽救生命的移植的生理健康器官和组织的来源，最近已被用于人类免疫学研究，通常仅限于外周血采样。捐献者包括不同的人群，在住院期间有不同的死亡原因和临床结果，这种变化对血液和组织中免疫参数的影响尚不清楚。在这里，我们对来自脑死亡器官捐赠者(3个月-93岁；n=291)的血液、淋巴(骨髓、脾、淋巴结)和粘膜(肺、肠)部位的先天性和获得性免疫成分进行了8项临床参数的协调分析。总体而言，献血者血液中单核细胞和淋巴细胞含量与健康对照组相似，血清促炎细胞因子水平较低；但献血者血液中性粒细胞和血清IL-8、IL-6和MCP-1水平升高，其水平因死因而异。在组织中，单核细胞、中性粒细胞、B淋巴细胞和T细胞亚群在淋巴或粘膜部位的频率和组成不随临床状态而变化，在供者中相似，与临床并发症的程度无关。我们的结果表明，器官捐赠者维持着组织的动态平衡，是人类免疫学基础研究的宝贵资源。
Organ donors are sources of physiologically healthy organs and tissues for life-saving transplantation, and have been recently used for human immunology studies which are typically confined to the sampling of peripheral blood. Donors comprise a diverse population with different causes of death and clinical outcomes during hospitalization, and the effects of such variations on immune parameters in blood and tissues are not known. We present here coordinate analyses of innate and adaptive immune components in blood, lymphoid (bone marrow, spleen, lymph nodes), and mucosal (lungs, intestines) sites from a population of brain-dead organ donors (3-months-93-years; n=291) across eight clinical parameters. Overall, the blood of donors exhibited similar monocyte and lymphocyte content and low serum levels of pro-inflammatory cytokines as healthy controls; however, donor blood had increased neutrophils and serum levels of IL-8, IL-6 and MCP-1 which varied with cause of death. In tissues, the frequency and composition of monocytes, neutrophils, B lymphocytes and T cell subsets in lymphoid or mucosal sites did not vary with clinical state, and was similar in donors independent of the extent of clinical complications. Our results reveal that organ donor maintain tissue homeostasis, and are a valuable resource for fundamental studies in human immunology.
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影响因子：
32.4
作者：
Sathaliyawala T;Kubota M;Yudanin N;Turner D;Camp P;Thome JJ;Bickham KL;Lerner H;Goldstein M;Sykes M;Kato T;Farber DL
通讯作者：
Farber DL
影响因子：
4.4
作者：
Havenith, Simone H. C.;Remmerswaal, Ester B. M.;van Lier, Rene A. W.
通讯作者：
van Lier, Rene A. W.
DOI：
10.1007/978-1-4939-2895-8_9
发表时间：
2015-01-01
期刊：
GLUCOCORTICOID SIGNALING: FROM MOLECULES TO MICE TO MAN
影响因子：
--
作者：
Oppong, Emmanuel;Cato, Andrew C. B.
通讯作者：
Cato, Andrew C. B.
DOI：
10.1097/01.ta.0000168708.78049.32
发表时间：
2005-05-01
影响因子：
--
作者：
Salim, A;Velmahos, GC;Demetriades, D
通讯作者：
Demetriades, D
影响因子：
2.8
作者：
Raza, Ali;Firasat, Sadaf;Abid, Aiysha
通讯作者：
Abid, Aiysha