Ultrastructure, synaptic organization, and molecular components of bushy cell networks in the anteroventral cochlear nucleus of the rhesus monkey.
Ultrastructure, synaptic organization, and molecular components of bushy cell networks in the anteroventral cochlear nucleus of the rhesus monkey.
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DOI：
10.1016/j.neuroscience.2011.01.058
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发表时间：
2011-04-14
期刊：
影响因子：
3.3
通讯作者：
Rubio, M. E.
中科院分区：
文献类型：
--
作者：
Gomez-Nieto, R.;Rubio, M. E.
关键词：
丛状细胞（BC）在耳蜗腹侧核（VCN）中处理听觉信息。然而，大多数神经解剖学发现来自猫和啮齿动物的研究，人类和高等非人灵长类动物中BC的超微结构形态特征尚不清楚。在这项研究中，我们结合组织学，免疫细胞化学和超微结构的方法来研究的形态和突触组织的猕猴VCN的BCs。我们观察到，BC被组织在一个复杂的神经网络中，似乎将细胞相互连接。BC胞体和树突的精细结构以及它们的突触输入与其他哺乳动物相似。我们发现，BCs接受了许多endbulb样的VGLUT 1和VGLUT 2免疫阳性的结束。此外，它们还表达谷氨酸AMPA（GluR 2/3和GluR 4）、NMDA（NR 1）、δ 1/2受体亚基和甘氨酸受体α1亚基。这些受体类型和亚基介导来自耳蜗的快速兴奋性突触传递和来自非耳蜗输入的抑制性神经传递。小清蛋白免疫染色和半薄切片显示，BC树突朝向相邻的BC胞体形成神经元簇。在簇内，传入的输入建立了多个不同的突触接触。因此，BCs通过专门的树体细胞和体细胞膜连接点连接。我们的研究结果表明，在灵长类动物和其他哺乳动物物种之间，BCs的细胞结构组织是非常保守的。
Bushy cells (BCs) process auditory information in the ventral cochlear nucleus (VCN). Yet, most neuroanatomical findings come from studies in cats and rodents, and the ultrastructural morphological features of BCs in humans and higher nonhuman primates are unknown. In this study, we combined histological, immunocytochemical, and ultrastructural methods to examine the morphology and synaptic organization of BCs in the rhesus monkey VCN. We observed that BCs were organized in a complex neural network that appears to interconnect the cells. The fine structure of BC somata and dendrites, as well as their synaptic inputs, are similar to those in other mammals. We found that BCs received numerous endbulb-like VGLUT1- and VGLUT2-immunopositive endings. In addition, they expressed glutamate AMPA (GluR2/3 and GluR4), NMDA (NR1), delta1/2 receptor subunits, and the α1 subunit of the glycine receptor. These receptor types and subunits mediate fast excitatory synaptic transmission from the cochlea and inhibitory neurotransmission from noncochlear inputs. Parvalbumin immunostaining and semithin sections showed that BC dendrites are oriented toward neighboring BC somas to form neuronal clusters. Within the cluster, the incoming inputs established multiple, divergent synaptic contacts. Thus, BCs were connected by specialized dendrosomatic and somasomatic membrane junctions. Our results indicate that the cytoarchitectural organization of BCs is well conserved between primates and other mammalian species.
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