Expression and functions of ASIC1 in the zebrafish retina.
Expression and functions of ASIC1 in the zebrafish retina.
复制标题
ASIC1 在斑马鱼视网膜中的表达和功能。
DOI：
10.1016/j.bbrc.2014.11.021
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Liu Chun-Feng
中科院分区：
文献类型：
--
作者：
Liu Sha;Wang Mei-Xia;Mao Cheng-Jie;Cheng Xiao-Yu;Wang Chen-Tao;Huang Jian;Zhong Zhao-Min;Hu Wei-Dong;Wang Fen;Hu Li-Fang;Wang Han;Liu Chun-Feng
研究表明，酸敏感离子通道存在于哺乳动物的中枢和外周神经系统，包括视网膜。然而，目前还不清楚斑马鱼的视网膜是否也表达ASIC。本研究检测了zasic1在斑马鱼视网膜中的表达和分布。在成年斑马鱼视网膜中检测到zasic1mRNA和蛋白的表达。整体原位杂交和免疫组织化学研究证实，ASIC1在斑马鱼视网膜中广泛分布。用全细胞膜片钳技术记录酸中毒诱发的视网膜神经节细胞(RGC)电流。此外，阻断ASICs通道显著减少了斑马鱼幼体对光照的运动反应。综上所述，我们的数据证明了ASIC1在斑马鱼视网膜中的存在及其可能的功能相关性。
It has been demonstrated that acid sensing ionic channels (ASICs) are present in the central and peripheral nervous system of mammals, including the retina. However, it remains unclear whether the zebrafish retina also expresses ASICs. In the present study, the expression and distribution ofzasic1were examined in the retina of zebrafish. Bothzasic1mRNA and protein expressions were detected in the adult zebrafish retina. A wide distribution of ASIC1 in zebrafish retina was confirmed using whole mount in situ hybridization and immunohistochemistry study. Acidosis-induced currents in the isolated retinal ganglion cells (RGCs) were also recorded using whole cell patch clamping. Moreover, blockade of ASICs channel significantly reduced the locomotion of larval zebrafish in response to light exposure. In sum, our data demonstrate the presence of ASIC1 and its possible functional relevance in the retina of zebrafish.
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影响因子：
34.7
作者：
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通讯作者：
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影响因子：
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影响因子：
16.2
作者：
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影响因子：
3.5
作者：
Li, Xiaoyu;Fei, Jianchun;Li, Jingxin
通讯作者：
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