Circulating micro-RNA expression profiles in early stage nonsmall cell lung cancer.
Circulating micro-RNA expression profiles in early stage nonsmall cell lung cancer.
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DOI：
10.1002/ijc.26153
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发表时间：
2012-03-15
影响因子：
6.4
通讯作者：
Harris, Curtis C.
中科院分区：
文献类型：
--
作者：
Heegaard, Niels H. H.;Schetter, Aaron J.;Welsh, Judith A.;Yoneda, Mitsuhiro;Bowman, Elise D.;Harris, Curtis C.
循环micro-RNA（miR）谱已被提出作为癌症（包括肺癌）的有希望的诊断和预后生物标志物。我们已经开发出准确和可重复测量血清和血浆中microRNA水平的方法。在这里，我们研究了220例早期NSCLC患者和220例匹配对照的配对血清和血浆样本。我们使用qRT-PCR来测量30种不同miR的循环水平，这些miR先前已被报道在肺癌组织中表达不同。对所有样品的10%进行重复RNA提取，并且这些重复样品之间的microRNA测量高度相关。这证明了我们的测定的高再现性。NSCLC患者血清中miR-146 b、miR-221、let-7a、miR-155、miR-17- 5 p、miR-27 a和miR-106 a的表达明显降低，而miR-29 c的表达明显升高。与对照组相比，患者血浆中未观察到显著差异。总体而言，血清中的表达水平与血浆中的水平没有很好的相关性。在次要分析中，let-7 B的血浆表达降低与所有患者中更差的癌症特异性死亡率适度相关，并且miR-223的血清表达降低与IA/B期患者中的癌症特异性死亡率适度相关。MiR配置文件也显示出相当大的差异比较非洲裔美国人和欧洲裔美国人。总之，当比较病例和对照时，我们发现miR表达存在显著差异，并发现let-7 b表达与NSCLC预后相关的证据。
Circulating micro-RNA (miR) profiles have been proposed as promising diagnostic and prognostic biomarkers for cancer, including lung cancer. We have developed methods to accurately and reproducibly measure microRNA levels in serum and plasma. Here we study paired serum and plasma samples from 220 patients with early stage NSCLC and 220 matched controls. We use qRT-PCR to measure the circulating levels of 30 different miRs that have previously been reported to be differently expressed in lung cancer tissue. Duplicate RNA extractions were performed for 10% of all samples and microRNA measurements were highly correlated among those duplicates. This demonstrates high reproducibility of our assay. The expression of miR-146b, miR-221, let-7a, miR-155, miR-17-5p, miR-27a and miR-106a were significantly reduced in the serum of NSCLC cases while miR-29c was significantly increased. No significant differences were observed in plasma of patients compared to controls. Overall, expression levels in serum did not correlate well with levels in plasma. In secondary analyses, reduced plasma expression of let-7b was modestly associated with worse cancer-specific mortality in all patients and reduced serum expression of miR-223 was modestly associated with cancer-specific mortality in stage IA/B patients. MiR profiles also showed considerable differences comparing African American and European Americans. In summary, we found significant differences in miR expression when comparing cases and controls and find evidence that expression of let-7b is associated with prognosis in NSCLC.
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DOI：
10.1158/1078-0432.ccr-09-1736
发表时间：
2010-01-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Landi MT;Zhao Y;Rotunno M;Koshiol J;Liu H;Bergen AW;Rubagotti M;Goldstein AM;Linnoila I;Marincola FM;Tucker MA;Bertazzi PA;Pesatori AC;Caporaso NE;McShane LM;Wang E
通讯作者：
Wang E
影响因子：
11.2
作者：
Nazarenko, Irina;Rana, Sanyukta;Zoeller, Margot
通讯作者：
Zoeller, Margot
影响因子：
6.5
作者：
Lawrie, Charles H.;Gal, Shira;Harris, Adrian L.
通讯作者：
Harris, Adrian L.
影响因子：
21.3
作者：
Skog, Johan;Wuerdinger, Tom;van Rijn, Sjoerd;Meijer, Dimphna H.;Gainche, Laura;Sena-Esteves, Miguel;Curry, William T., Jr.;Carter, Bob S.;Krichevsky, Anna M.;Breakefield, Xandra O.
通讯作者：
Breakefield, Xandra O.
影响因子：
50.3
作者：
Garofalo M;Di Leva G;Romano G;Nuovo G;Suh SS;Ngankeu A;Taccioli C;Pichiorri F;Alder H;Secchiero P;Gasparini P;Gonelli A;Costinean S;Acunzo M;Condorelli G;Croce CM
通讯作者：
Croce CM