Bacterial Community Members Increase Bacillus subtilis Maintenance on the Roots of Arabidopsis thaliana.
Bacterial Community Members Increase Bacillus subtilis Maintenance on the Roots of Arabidopsis thaliana.
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DOI：
10.1094/pbiomes-02-20-0019-r
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发表时间：
2020
影响因子：
4.4
通讯作者：
Shank EA
中科院分区：
文献类型：
--
作者：
Eckshtain-Levi N;Harris SL;Roscios RQ;Shank EA
植物促生长细菌（PGPB）用于改善植物健康并促进作物生产。然而，由于一些 PGPB（包括枯草芽孢杆菌）随着时间的推移不会在植物根部保持大量定殖，因此尚不清楚 PGPB 在整个植物生长周期中的有效性如何。为了开发和利用 PGPB 的潜力，需要更好地了解植物根群落组装的动态。尽管枯草芽孢杆菌通常是根部微生物组的成员，但它不能有效地与植物根部单结合。我们假设枯草芽孢杆菌可能需要其他主要定殖者才能有效地与植物根部结合。我们利用先前设计的水培系统将细菌添加到拟南芥根中，并随着时间的推移监测它们的附着情况。我们用枯草芽孢杆菌和来自本地拟南芥根部微生物组的单个细菌分离物单独或一起接种于幼苗。然后我们测量了共菌体如何影响枯草芽孢杆菌在拟南芥根上定殖和维持的能力。我们筛选了 96 个完全基因组测序的菌株，并鉴定了 5 个能够显着改善枯草芽孢杆菌维持的细菌菌株。其中三种根际细菌还增加了两种解淀粉芽孢杆菌菌株的维持，这两种菌株通常用于市售生物添加剂。这些结果不仅说明了该模型系统在解决有关植物-微生物相互作用以及其他细菌如何影响 PGPB 维持与植物根关系的能力的问题方面的实用性，而且还可能有助于为未来的农业干预措施提供信息，以提高作物产量。
Plant-growth-promoting bacteria (PGPB) are used to improve plant health and promote crop production. However, because some PGPB (including Bacillus subtilis) do not maintain substantial colonization on plant roots over time, it is unclear how effective PGPB are throughout the plant growing cycle. A better understanding of the dynamics of plant root community assembly is needed to develop and harness the potential of PGPB. Although B. subtilis is often a member of the root microbiome, it does not efficiently monoassociate with plant roots. We hypothesized that B. subtilis may require other primary colonizers to efficiently associate with plant roots. We utilized a previously designed hydroponic system to add bacteria to Arabidopsis thaliana roots and monitor their attachment over time. We inoculated seedlings with B. subtilis and individual bacterial isolates from the native A. thaliana root microbiome either alone or together. We then measured how the coinoculum affected the ability of B. subtilis to colonize and maintain on A. thaliana roots. We screened 96 fully genome-sequenced strains and identified five bacterial strains that were able to significantly improve the maintenance of B. subtilis. Three of these rhizobacteria also increased the maintenance of two strains of B. amyloliquefaciens commonly used in commercially available bioadditives. These results not only illustrate the utility of this model system to address questions about plant–microbe interactions and how other bacteria affect the ability of PGPB to maintain their relationships with plant roots but also may help inform future agricultural interventions to increase crop yields.
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影响因子：
9.8
作者：
Busby PE;Soman C;Wagner MR;Friesen ML;Kremer J;Bennett A;Morsy M;Eisen JA;Leach JE;Dangl JL
通讯作者：
Dangl JL
影响因子：
6.4
作者：
Abisado RG;Benomar S;Klaus JR;Dandekar AA;Chandler JR
通讯作者：
Chandler JR
影响因子：
9.8
作者：
Finkel, Omri M.;Salas-Gonzalez, Isai;Dangl, Jeffery L.
通讯作者：
Dangl, Jeffery L.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
从自然环境中分离的枯草芽孢杆菌对番茄枯萎病的生物防治取决于介导生物膜形成的保守基因。
影响因子：
5.1
作者：
Chen Y;Yan F;Chai Y;Liu H;Kolter R;Losick R;Guo JH
通讯作者：
Guo JH