Multiple Sox genes are expressed in stem cells or in differentiating neuro-sensory cells in the hydrozoan Clytia hemisphaerica.
Multiple Sox genes are expressed in stem cells or in differentiating neuro-sensory cells in the hydrozoan Clytia hemisphaerica.
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DOI：
10.1186/2041-9139-2-12
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发表时间：
2011-06-01
期刊：
影响因子：
4.1
通讯作者：
Manuel M
中科院分区：
文献类型：
--
作者：
Jager M;Quéinnec E;Le Guyader H;Manuel M
Sox基因是动物发育的重要调节因子，属于含HMG结构域的转录因子类。双侧模型中的研究已经显著地突出了它们在控制沿着细胞谱系的进展中的关键作用，各种Sox家族成员参与自我更新干细胞/增殖祖细胞与经历分化的细胞之间的关键平衡的一侧或另一侧。我们研究了10个Sox基因在刺胞动物Clytia hemisphaerica中的表达。我们的系统发育分析将这些Clytia基因中的大部分分配到先前确定的Sox组：SoxB（CheSox 2，CheSox 3，CheSox 10，CheSox 13，CheSox 14），SoxC（CheSox 12），SoxE（CheSox 1，CheSox 5）和SoxF（CheSox 11），一个基因（CheSox 15）仍然未分类。在浮浪幼体和水母中，SoxF直向同源物在整个内胚层中表达。其他基因在干细胞/未分化的祖细胞中表达，或在具有神经感觉特性的分化（-艾德）细胞（神经细胞或神经元）中表达。此外，它们中的大多数在雌性生殖系中表达，它们的母体转录本要么定位于卵的动物区域，要么均匀分布。与其他刺胞动物相比，栉水母和双侧面动物在Sox家族水平上的基因表达/功能的某些方面显示了古老的进化保守性：（i）许多Sox基因在干细胞和/或未分化的祖细胞中表达;（ii）其他基因，或在不同的背景下相同的基因，与神经感觉细胞的分化有关;（iii）Sox基因通常在生殖细胞中表达;（iv）SoxF组基因与内胚层衍生物相关。引人注目的是，在给定的Sox直系组和干细胞/祖细胞中的表达/功能与分化细胞中的表达/功能之间完全缺乏相关性，这意味着Sox基因可以在进化过程中容易地从这些基本细胞状态之间的平衡的一侧切换到另一侧。
The Sox genes are important regulators of animal development belonging to the HMG domain-containing class of transcription factors. Studies in bilaterian models have notably highlighted their pivotal role in controlling progression along cell lineages, various Sox family members being involved at one side or the other of the critical balance between self-renewing stem cells/proliferating progenitors, and cells undergoing differentiation. We have investigated the expression of 10 Sox genes in the cnidarian Clytia hemisphaerica. Our phylogenetic analyses allocated most of these Clytia genes to previously-identified Sox groups: SoxB (CheSox2, CheSox3, CheSox10, CheSox13, CheSox14), SoxC (CheSox12), SoxE (CheSox1, CheSox5) and SoxF (CheSox11), one gene (CheSox15) remaining unclassified. In the planula larva and in the medusa, the SoxF orthologue was expressed throughout the endoderm. The other genes were expressed either in stem cells/undifferentiated progenitors, or in differentiating (-ed) cells with a neuro-sensory identity (nematocytes or neurons). In addition, most of them were expressed in the female germline, with their maternal transcripts either localised to the animal region of the egg, or homogeneously distributed. Comparison with other cnidarians, ctenophores and bilaterians suggest ancient evolutionary conservation of some aspects of gene expression/function at the Sox family level: (i) many Sox genes are expressed in stem cells and/or undifferentiated progenitors; (ii) other genes, or the same under different contexts, are associated with neuro-sensory cell differentiation; (iii) Sox genes are commonly expressed in the germline; (iv) SoxF group genes are associated with endodermal derivatives. Strikingly, total lack of correlation between a given Sox orthology group and expression/function in stem cells/progenitors vs. in differentiating cells implies that Sox genes can easily switch from one side to the other of the balance between these fundamental cellular states in the course of evolution.
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