Human tissue distribution of TA02, which is homologous with a new type of aspartic proteinase, napsin A.
Human tissue distribution of TA02, which is homologous with a new type of aspartic proteinase, napsin A.
复制标题
DOI：
10.1111/j.1349-7006.2000.tb00879.x
复制
发表时间：
2000-10
期刊：
影响因子：
--
通讯作者：
Kato H
中科院分区：
文献类型：
--
作者：
Hirano T;Auer G;Maeda M;Hagiwara Y;Okada S;Ohira T;Okuzawa K;Fujioka K;Franzén B;Hibi N;Seito T;Ebihara Y;Kato H
测定了与原发性肺腺癌相关的TA 02（分子量35.0 kDa，等电点5.29）的N-末端氨基酸序列，并使用片段肽生成针对TA 02的小鼠单克隆抗体（mAb）。氨基酸序列分析表明，TA 02可能与一种新型天冬氨酸蛋白酶napsin A同源。在这种情况下，我们使用逆转录聚合酶链反应（RT-PCR）证实了napsin A在原发性肺腺癌中的表达，并显示TA 02 mAb与谷胱甘肽-S-转移酶（GST）-napsin A融合蛋白反应。我们的结论是，TA 02是相同的分子，并显示其主要分布在II型肺泡细胞，肺泡巨噬细胞，肾小管和胰腺外分泌腺和导管。特别地，II型肺细胞和肺泡巨噬细胞在人正常组织中显示出TA 02的高表达。原发性肺腺癌58个病灶中47个（81.0%）为阳性。除杯状细胞型腺癌外，所有高分化腺癌均显示TA 02的高表达。此外，7例大细胞癌中有2例（28.6%）显示TA 02低表达。其他组织病理学类型的原发性肺癌完全不表达TA 02。少数肾细胞癌、胰腺癌、乳腺癌、甲状腺癌、结肠癌和卵巢癌呈低表达，但染色模式与原发性肺腺癌完全不同，表现为颗粒状染色模式。我们的新单克隆抗体对TA 02/napsin A的免疫化学检测具有重要价值。
The N‐terminal amino acid sequence of TA02 (molecular weight 35.0 kDa, isoelectric point 5.29), which is associated with primary lung adenocarcinoma, was determined and a fragment peptide was used to generate mouse monoclonal antibodies (mAbs) against TA02. The amino acid sequence suggested that TA02 might be homologous with napsin A, a new type of aspartic proteinase. In this context, we confirmed the expression of napsin A in primary lung adenocarcinoma using reversetranscription polymerare chain reaction (RT‐PCR) and showed that the TA02 mAbs reacted with glutathione‐S‐transferase (GST)‐napsin A fusion protein. We concluded that TA02 is the same molecule as napsin A, and showed immunohistochemically that it is distributed mainly in type II pneumocytes, alveolar macrophages, renal tubules and exocrine glands and ducts in the pancreas. In particular, type II pneumocytes and alveolar macrophages showed high expression of TA02 among human normal tissues. In primary lung adenocarcinoma, 47 out of 58 (81.0%) primary lesions were positive. All well‐differentiated adenocarcinomas except those of goblet cell type showed high expression of TA02. In addition, two out of seven (28.6%) large cell carcinomas showed low expression of TA02. The other histopathological types of primary lung cancer did not express TA02 at all. A few cases of renal cell cancer, pancreatic cancer, breast cancer, thyroid cancer, colon cancer and ovarian cancer showed low expression, but the staining patterns were completely different from that of primary lung adenocarcinoma, which showed a granular staining pattern. Our novel mAbs should be valuable for immunochemical detection of TA02/napsin A.
登录
查看更多内容
影响因子：
8.8
作者：
HIRANO, T;FRANZEN, B;AUER, G
通讯作者：
AUER, G
DOI：
10.1165/ajrcmb/3.5.491
发表时间：
1990-11-01
影响因子：
6.4
作者：
AUTEN, RL;WATKINS, RH;HOROWITZ, S
通讯作者：
HOROWITZ, S
DOI：
10.1111/j.1349-7006.1994.tb02969.x
发表时间：
1994-09-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
BANNO, S;YOSHIKAWA, K;SETO, M
通讯作者：
SETO, M
影响因子：
2.9
作者：
OKUZAWA, K;FRANZEN, B;AUER, G
通讯作者：
AUER, G
影响因子：
64.8
作者：
LAEMMLI, UK
通讯作者：
LAEMMLI, UK