Potent cross-reactive antibodies following Omicron breakthrough in vaccinees.
Potent cross-reactive antibodies following Omicron breakthrough in vaccinees.
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DOI：
10.1016/j.cell.2022.05.014
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发表时间：
2022-06-09
期刊：
影响因子：
64.5
通讯作者：
Screaton, Gavin R.
中科院分区：
文献类型：
--
作者：
Nutalai, Rungtiwa;Zhou, Daming;Tuekprakhon, Aekkachai;Ginn, Helen M.;Supasa, Piyada;Liu, Chang;Huo, Jiandong;Mentzer, Alexander J.;Duyvesteyn, Helen M. E.;Dijokaite-Guraliuc, Aiste;Skelly, Donal;Ritter, Thomas G.;Amini, Ali;Bibi, Sagida;Adele, Sandra;Johnson, Sile Ann;Constantinides, Bede;Webster, Hermione;Temperton, Nigel;Klenerman, Paul;Barnes, Eleanor;Dunachie, Susanna J.;Crook, Derrick;Pollard, Andrew J.;Lambe, Teresa;Goulder, Philip;Paterson, Neil G.;Williams, Mark A.;Hall, David R.;Mongkolsapaya, Juthathip;Fry, Elizabeth E.;Dejnirattisai, Wanwisa;Ren, Jingshan;Stuart, David, I;Screaton, Gavin R.
SARS-CoV-2的高传播性Omicron变体目前在全球占主导地位。在这里，我们比较了Omicron BA.1、BA.1.1和BA.2的中和作用。BA.2 RBD具有比BA.1略高的ACE 2亲和力和略低的疫苗血清中和作用，可能与其增加的传播性相关。mAb（包括治疗剂）亚系之间的中和差异主要源于ACE 2结合位点边界残基的变化;然而，更远的突变S371 F（BA.2）和R346 K（BA.1.1）显著降低治疗性抗体Vir-S309的中和作用。对从接种疫苗的志愿者中分离的27种有效的RBD结合mAb进行深入的结构和功能分析，发现它们集中在RBD内的两个主要簇中，其中有效的右肩抗体显示出患病率增加。选择和体细胞成熟在较少突变的表位中具有优化的抗体效力，并且在高度突变的表位中具有恢复的效力。所有27种单克隆抗体都能有效地中和早期大流行毒株，许多单克隆抗体对相关变异株表现出广泛的反应性。来自Omicron突破疫苗的有效RBD抗体广泛中和VoC。这些可能的回忆抗体集中在两个主要簇中。体细胞成熟适应公共抗体以恢复效力BA.2 > BA.1 ACE 2亲和力。BA.2 < BA.1疫苗血清和Vir-S309的中和作用对SARS-CoV-2 Omicron突破性感染的抗体的分析揭示了它们的结构和功能特性以及中和不同大流行毒株的能力。
Highly transmissible Omicron variants of SARS-CoV-2 currently dominate globally. Here, we compare neutralization of Omicron BA.1, BA.1.1, and BA.2. BA.2 RBD has slightly higher ACE2 affinity than BA.1 and slightly reduced neutralization by vaccine serum, possibly associated with its increased transmissibility. Neutralization differences between sub-lineages for mAbs (including therapeutics) mostly arise from variation in residues bordering the ACE2 binding site; however, more distant mutations S371F (BA.2) and R346K (BA.1.1) markedly reduce neutralization by therapeutic antibody Vir-S309. In-depth structure-and-function analyses of 27 potent RBD-binding mAbs isolated from vaccinated volunteers following breakthrough Omicron-BA.1 infection reveals that they are focused in two main clusters within the RBD, with potent right-shoulder antibodies showing increased prevalence. Selection and somatic maturation have optimized antibody potency in less-mutated epitopes and recovered potency in highly mutated epitopes. All 27 mAbs potently neutralize early pandemic strains, and many show broad reactivity with variants of concern. Potent RBD antibodies from Omicron breakthrough vaccinees broadly neutralize VoC These, possible recall antibodies, are focused in two main clusters Somatic maturation adapts public antibodies to recover potency BA.2 > BA.1 ACE2 affinity. BA.2 < BA.1 neutralization by vaccine serum and Vir-S309 Analysis of antibodies from SARS-CoV-2 Omicron breakthrough infections reveals their structural and functional properties as well as ability to neutralize different pandemic strains.
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影响因子：
64.5
作者：
Dejnirattisai W;Zhou D;Ginn HM;Duyvesteyn HME;Supasa P;Case JB;Zhao Y;Walter TS;Mentzer AJ;Liu C;Wang B;Paesen GC;Slon-Campos J;López-Camacho C;Kafai NM;Bailey AL;Chen RE;Ying B;Thompson C;Bolton J;Fyfe A;Gupta S;Tan TK;Gilbert-Jaramillo J;James W;Knight M;Carroll MW;Skelly D;Dold C;Peng Y;Levin R;Dong T;Pollard AJ;Knight JC;Klenerman P;Temperton N;Hall DR;Williams MA;Paterson NG;Bertram FKR;Siebert CA;Clare DK;Howe A;Radecke J;Song Y;Townsend AR;Huang KA;Fry EE;Mongkolsapaya J;Diamond MS;Ren J;Stuart DI;Screaton GR
通讯作者：
Screaton GR
影响因子：
64.8
作者：
Andreano E;Paciello I;Piccini G;Manganaro N;Pileri P;Hyseni I;Leonardi M;Pantano E;Abbiento V;Benincasa L;Giglioli G;De Santi C;Fabbiani M;Rancan I;Tumbarello M;Montagnani F;Sala C;Montomoli E;Rappuoli R
通讯作者：
Rappuoli R
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1056/nejmoa2029849
发表时间：
2021-01-21
期刊：
The New England journal of medicine
影响因子：
--
作者：
Chen P;Nirula A;Heller B;Gottlieb RL;Boscia J;Morris J;Huhn G;Cardona J;Mocherla B;Stosor V;Shawa I;Adams AC;Van Naarden J;Custer KL;Shen L;Durante M;Oakley G;Schade AE;Sabo J;Patel DR;Klekotka P;Skovronsky DM;BLAZE-1 Investigators
通讯作者：
BLAZE-1 Investigators
影响因子：
64.8
作者：
Cho A;Muecksch F;Schaefer-Babajew D;Wang Z;Finkin S;Gaebler C;Ramos V;Cipolla M;Mendoza P;Agudelo M;Bednarski E;DaSilva J;Shimeliovich I;Dizon J;Daga M;Millard KG;Turroja M;Schmidt F;Zhang F;Tanfous TB;Jankovic M;Oliveria TY;Gazumyan A;Caskey M;Bieniasz PD;Hatziioannou T;Nussenzweig MC
通讯作者：
Nussenzweig MC