Persistent up-regulation of polyribosomes at synapses during long-term memory, reconsolidation, and extinction of associative memory.
Persistent up-regulation of polyribosomes at synapses during long-term memory, reconsolidation, and extinction of associative memory.
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DOI：
10.1101/lm.053577.122
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发表时间：
2022-08
影响因子：
2
通讯作者：
Cain, Christopher K.
中科院分区：
文献类型：
--
作者：
Ostroff, Linnaea E.;Cain, Christopher K.
突触的局部蛋白质合成可以提供快速的蛋白质供应，以支持新记忆巩固过程中的突触变化，但其在维持或更新已建立的记忆中的作用尚不清楚。巩固需要在学习后立即合成新的蛋白质，而已建立的记忆则对蛋白质合成抑制剂有抵抗力。我们之前报道过，在厌恶性巴甫洛夫条件反射的巩固过程中，多核糖体在外侧杏仁核（LA）中上调。在这项研究中，我们使用连续切片电镜重建来确定树突状多核糖体的分布是否在长期记忆阶段恢复到基线。与对照组相比，长期记忆与树突（包括各种大小的树突棘）中多核糖体的上调有关。通过呈现少量线索来检索巩固的记忆，会引起对蛋白质合成的新的、短暂的需求来维持记忆，而呈现大量线索会导致消退学习，形成新的记忆。在恢复或消失训练1小时后，树突多核糖体的分布相似，但在最小的棘中，消失组的多核糖体较多。我们的研究结果表明，学习对树突状多核糖体的影响并不局限于记忆形成的瞬时翻译依赖阶段。暗示的巴甫洛夫条件反射诱导了LA中持续的突触增强，而这种增强不会被恢复或消失所逆转，因此树突状多核糖体可能与突触强度普遍相关，而不是与近期活动或短暂的翻译过程相关。
Local protein synthesis at synapses can provide a rapid supply of proteins to support synaptic changes during consolidation of new memories, but its role in the maintenance or updating of established memories is unknown. Consolidation requires new protein synthesis in the period immediately following learning, whereas established memories are resistant to protein synthesis inhibitors. We have previously reported that polyribosomes are up-regulated in the lateral amygdala (LA) during consolidation of aversive-cued Pavlovian conditioning. In this study, we used serial section electron microscopy reconstructions to determine whether the distribution of dendritic polyribosomes returns to baseline during the long-term memory phase. Relative to control groups, long-term memory was associated with up-regulation of polyribosomes throughout dendrites, including in dendritic spines of all sizes. Retrieval of a consolidated memory by presentation of a small number of cues induces a new, transient requirement for protein synthesis to maintain the memory, while presentation of a large number of cues results in extinction learning, forming a new memory. One hour after retrieval or extinction training, the distribution of dendritic polyribosomes was similar except in the smallest spines, which had more polyribosomes in the extinction group. Our results demonstrate that the effects of learning on dendritic polyribosomes are not restricted to the transient translation-dependent phase of memory formation. Cued Pavlovian conditioning induces persistent synapse strengthening in the LA that is not reversed by retrieval or extinction, and dendritic polyribosomes may therefore correlate generally with synapse strength as opposed to recent activity or transient translational processes.
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DOI：
10.1002/cne.25158
发表时间：
2021-08-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Gindina S;Botsford B;Cowansage K;LeDoux J;Klann E;Hoeffer C;Ostroff L
通讯作者：
Ostroff L
影响因子：
16.2
作者：
Duvarci S;Pare D
通讯作者：
Pare D
DOI：
10.1037/0097-7403.29.4.323
发表时间：
2003-10-01
期刊：
JOURNAL OF EXPERIMENTAL PSYCHOLOGY-ANIMAL BEHAVIOR PROCESSES
影响因子：
--
作者：
Cain, CK;Blouin, AM;Barad, M
通讯作者：
Barad, M
影响因子：
3.4
作者：
Duvarci, Sevil;ben Mamou, Cyrinne;Nader, Karim
通讯作者：
Nader, Karim
DOI：
10.1126/science.1242939
发表时间：
2014-01-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Buxbaum AR;Wu B;Singer RH
通讯作者：
Singer RH