Oral pathobiont induces systemic inflammation and metabolic changes associated with alteration of gut microbiota.
Oral pathobiont induces systemic inflammation and metabolic changes associated with alteration of gut microbiota.
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DOI：
10.1038/srep04828
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发表时间：
2014-05-06
影响因子：
4.6
通讯作者：
Yamazaki K
中科院分区：
文献类型：
--
作者：
Arimatsu K;Yamada H;Miyazawa H;Minagawa T;Nakajima M;Ryder MI;Gotoh K;Motooka D;Nakamura S;Iida T;Yamazaki K
牙周炎已被认为是代谢紊乱的危险因素，如2型糖尿病、动脉粥样硬化性血管疾病和非酒精性脂肪肝。虽然牙菌斑的细菌感染和/或发炎牙龈产生的循环炎性细胞因子升高是牙周炎与这些疾病联系的可疑机制，但缺乏直接证据。我们推测，吞咽细菌对肠道微生物群的干扰诱导代谢性内毒素血症，导致代谢紊乱。为了研究这一假设，在小鼠口服牙龈卟啉单胞菌（一种代表性的牙周病原体）后，分析了肠道微生物群、胰岛素和葡萄糖耐受不良以及组织炎症水平的变化。焦磷酸测序显示，在施用牙龈卟啉单胞菌的小鼠中，属于拟杆菌目的群体显著升高，这与胰岛素抵抗和全身性炎症的增加相一致。在给予牙龈卟啉单胞菌的小鼠中，血液内毒素水平倾向于更高，而回肠中紧密连接蛋白的基因表达显著降低。这些结果为牙周炎与全身性疾病的相互关系提供了一个新的范例。
Periodontitis has been implicated as a risk factor for metabolic disorders such as type 2 diabetes, atherosclerotic vascular diseases, and non-alcoholic fatty liver disease. Although bacteremias from dental plaque and/or elevated circulating inflammatory cytokines emanating from the inflamed gingiva are suspected mechanisms linking periodontitis and these diseases, direct evidence is lacking. We hypothesize that disturbances of the gut microbiota by swallowed bacteria induce a metabolic endotoxemia leading metabolic disorders. To investigate this hypothesis, changes in the gut microbiota, insulin and glucose intolerance, and levels of tissue inflammation were analysed in mice after oral administration of Porphyromonas gingivalis, a representative periodontopathogens. Pyrosequencing revealed that the population belonging to Bacteroidales was significantly elevated in P. gingivalis-administered mice which coincided with increases in insulin resistance and systemic inflammation. In P. gingivalis-administered mice blood endotoxin levels tended to be higher, whereas gene expression of tight junction proteins in the ileum was significantly decreased. These results provide a new paradigm for the interrelationship between periodontitis and systemic diseases.
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影响因子：
4.8
作者：
Bahekar, Amol Ashok;Singh, Sarabjeet;Arora, Rohit
通讯作者：
Arora, Rohit
影响因子：
6.7
作者：
Ide, M;McPartlin, D;Wilson, RF
通讯作者：
Wilson, RF
影响因子：
3.5
作者：
Hujoel, PP;White, BA;Listgarten, MA
通讯作者：
Listgarten, MA
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars