Examination of Endogenous Peptides in Medicago truncatula Using Mass Spectrometry Imaging.
Examination of Endogenous Peptides in Medicago truncatula Using Mass Spectrometry Imaging.
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DOI：
10.1021/acs.jproteome.6b00471
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发表时间：
2016-12-02
影响因子：
4.4
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Gemperline E;Keller C;Jayaraman D;Maeda J;Sussman MR;Ané JM;Li L
植物科学是一个重要的、迅速发展的研究领域。在植物科学中，随着最近的技术进步（如质谱法）而迅速发展的一个研究领域是植物组学领域;然而，与蛋白质组学和代谢组学相比，植物肽组学相对不发达。内源性植物肽可以作为信号分子，并已被证明影响细胞分裂，发育，生长，繁殖，共生协会和防御反应。越来越需要在分子水平上揭示内源性肽的作用。质谱成像（MSI）是生物分析的一种有价值的工具，因为它允许在单个实验中检测数千种分析物，并且还显示检测到的分析物的空间信息。尽管预测了大量的植物肽，但目前缺乏具有空间定位和化学特异性的检测和成像。本文采用基质辅助激光解吸电离（MALDI）-MSI技术对蒺藜苜蓿内源肽和蛋白质进行了分析。数百个内源肽和蛋白质片段的成像，与有趣的肽空间分布的变化观察到植物在不同的发展阶段。
Plant science is an important, rapidly developing area of study. Within plant science, one area of study that has grown tremendously with recent technological advances, such as mass spectrometry, is the field of plant-omics; however, plant peptidomics is relatively underdeveloped in comparison with proteomics and metabolomics. Endogenous plant peptides can act as signaling molecules and have been shown to affect cell division, development, nodulation, reproduction, symbiotic associations, and defense reactions. There is a growing need to uncover the role of endogenous peptides on a molecular level. Mass spectrometric imaging (MSI) is a valuable tool for biological analyses as it allows for the detection of thousands of analytes in a single experiment and also displays spatial information for the detected analytes. Despite the prediction of a large number of plant peptides, their detection and imaging with spatial localization and chemical specificity is currently lacking. Here we analyzed the endogenous peptides and proteins in Medicago truncatula using matrix-assisted laser desorption/ionization (MALDI)–MSI. Hundreds of endogenous peptides and protein fragments were imaged, with interesting peptide spatial distribution changes observed between plants in different developmental stages.
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