Isolation of cellulolytic bacteria from the intestine of Diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass.
Isolation of cellulolytic bacteria from the intestine of Diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass.
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DOI：
10.1186/s13568-015-0101-z
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Castagnaro AP
中科院分区：
文献类型：
--
作者：
Dantur KI;Enrique R;Welin B;Castagnaro AP
为了寻找高效的木质纤维素降解酶/微生物用于甘蔗生物量的预处理，从取食甘蔗二化蛾幼虫的肠道中分离到118株可培养的细菌。用可溶性羧甲基纤维素(CMC)降解法或以甘蔗菌体为唯一碳源测定了所有菌株的纤维分解活性。对分离到的38株菌株进行了16S rDNA序列的系统发育分析，结果表明，所有的纤维素降解菌株都属于γ门-变形杆菌、放线杆菌和纤维素门。在这三个门中，鉴定出属于五个不同属的物种(克雷伯氏菌、营养单胞菌、微杆菌、芽孢杆菌和肠球菌)。芽孢杆菌属和克雷伯氏菌属细菌在甘蔗生物量上的生长以及在可溶性纤维素上诱导的胞外葡聚糖酶活性最高。较好的纤维分解活性与较高的胞外蛋白浓度相关。此外，扫描显微镜研究显示，纤维素分解菌株附着在不同的甘蔗基质上。本研究的结果表明，从取食甘蔗的昆虫幼虫肠道中寻找高效的纤维素降解酶和微生物的可能性及其在甘蔗生物质的工业化加工中的应用，如第二代生物燃料生产。
As a strategy to find efficient lignocellulose degrading enzymes/microorganisms for sugarcane biomass pretreatment purposes, 118 culturable bacterial strains were isolated from intestines of sugarcane-fed larvae of the moth Diatraea saccharalis. All strains were tested for cellulolytic activity using soluble carboxymethyl cellulose (CMC) degrading assays or by growing bacteria on sugarcane biomass as sole carbon sources. Out of the 118 strains isolated thirty eight were found to possess cellulose degrading activity and phylogenetic studies of the 16S rDNA sequence revealed that all cellulolytic strains belonged to the phyla γ-Proteobacteria, Actinobacteria and Firmicutes. Within the three phyla, species belonging to five different genera were identified (Klebsiella, Stenotrophomonas, Microbacterium, Bacillus and Enterococcus). Bacterial growth on sugarcane biomass as well as extracellular endo-glucanase activity induced on soluble cellulose was found to be highest in species belonging to genera Bacillus and Klebsiella. Good cellulolytic activity correlated with high extracellular protein concentrations. In addition, scanning microscopy studies revealed attachment of cellulolytic strains to different sugarcane substrates. The results of this study indicate the possibility to find efficient cellulose degrading enzymes and microorganisms from intestines of insect larvae feeding on sugarcane and their possible application in industrial processing of sugarcane biomass such as second generation biofuel production.
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影响因子：
2.8
作者：
Charrier, M;Combet-Blanc, Y;Ollivier, B
通讯作者：
Ollivier, B
影响因子：
--
作者：
Canilha L;Kumar Chandel A;dos Santos Milessi TS;Fernandes Antunes FA;da Costa Freitas WL;das Graças Almeida Felipe M;da Silva SS
通讯作者：
da Silva SS
DOI：
10.1016/j.cbpb.2004.07.006
发表时间：
2004-09-01
影响因子：
2.3
作者：
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通讯作者：
Sripathi, K
影响因子：
6.7
作者：
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通讯作者：
ANDANT, GERARD
影响因子：
5.6
作者：
Huang S;Sheng P;Zhang H
通讯作者：
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