Isolation and identification of cellulolytic bacteria from the gut of Holotrichia parallela larvae (Coleoptera: Scarabaeidae).
Isolation and identification of cellulolytic bacteria from the gut of Holotrichia parallela larvae (Coleoptera: Scarabaeidae).
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DOI：
10.3390/ijms13032563
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发表时间：
2012
影响因子：
5.6
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Huang S;Sheng P;Zhang H
本研究从大黑鳃金龟（Holotrichia parallela）幼虫肠道中分离到207株好氧和兼性厌氧纤维素分解菌。通过扩增核糖体DNA限制性分析（ARMRNA），这些细菌分离物被分配到21个基因型。部分16S rDNA序列分析和标准的生化和生理测试用于分配的21个代表性的菌株。结果表明，纤维素分解菌以变形菌门（Proteobacteria）占优势（70.05%），其次是放线菌门（Actinobacteria）（24.15%）、厚壁菌门（Firmicutes）（4.35%）和拟杆菌门（Bacteroidetes）（1.45%）。在属的水平上，革兰氏阴性菌属（Pseudomonas）、苍白杆菌属（Ochrobactrum）、根瘤菌属（Rhizobium）、纤维微菌属（Cellulosimicrobium）和微杆菌属（Microbacterium）是优势菌群，但也发现了芽孢杆菌属（Bacillus）、双枝菌属（Dyadenium）、管菌属（Siphonophylum）、副球菌属（Paracoccus）、Kaistia、Devosia、Labrys、Ensifer、Variovorax、Shinella、柠檬酸杆菌属（Citrobacter）和寡养单胞菌属（Stenotrophomonas）等成员。并首次报道了水斑虹吸管菌、Cellulosimicrobium funkei、Paracoccus sulfuroxidans、Ochrobactrum cytisi、Ochrobactrum haematophilum、Kaistia adipata、Devosia riboflavina、Labrys neptuniae、Ensifer adhaerens、Shinella zoogloeoides、Citrobacter freundii和Pseudomonas nitroreducens具有纤维素分解活性。我们的研究结果表明，圣甲虫肠道是一个有吸引力的来源，用于纤维素降解的新型纤维素分解微生物和酶的研究。
In this study, 207 strains of aerobic and facultatively anaerobic cellulolytic bacteria were isolated from the gut of Holotrichia parallela larvae. These bacterial isolates were assigned to 21 genotypes by amplified ribosomal DNA restriction analysis (ARDRA). A partial 16S rDNA sequence analysis and standard biochemical and physiological tests were used for the assignment of the 21 representative isolates. Our results show that the cellulolytic bacterial community is dominated by the Proteobacteria (70.05%), followed by the Actinobacteria (24.15%), the Firmicutes (4.35%), and the Bacteroidetes (1.45%). At the genus level, Gram-negative bacteria including Pseudomonas, Ochrobactrum, Rhizobium, Cellulosimicrobium, and Microbacterium were the predominant groups, but members of Bacillus, Dyadobacter, Siphonobacter, Paracoccus, Kaistia, Devosia, Labrys, Ensifer, Variovorax, Shinella, Citrobacter, and Stenotrophomonas were also found. Furthermore, our results suggest that a significant amount of bacterial diversity exists among the cellulolytic bacteria, and that Siphonobacter aquaeclarae, Cellulosimicrobium funkei, Paracoccus sulfuroxidans, Ochrobactrum cytisi, Ochrobactrum haematophilum, Kaistia adipata, Devosia riboflavina, Labrys neptuniae, Ensifer adhaerens, Shinella zoogloeoides, Citrobacter freundii, and Pseudomonas nitroreducens are reported to be cellulolytic for the first time in this study. Our results indicate that the scarab gut is an attractive source for the study of novel cellulolytic microorganisms and enzymes useful for cellulose degradation.
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影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
3.6
作者：
Cazemier, AE;Hackstein, JHP;vanderDrift, C
通讯作者：
vanderDrift, C
影响因子：
3.9
作者：
Ferreira, Susana;Duarte, Ana P.;Domingues, Fernanda C.
通讯作者：
Domingues, Fernanda C.
影响因子：
6
作者：
Hamelinck, CN;van Hooijdonk, G;Faaij, APC
通讯作者：
Faaij, APC
影响因子：
4.4
作者：
Egert, M;Stingl, U;Friedrich, MW
通讯作者：
Friedrich, MW