Mechanisms of Wharton's Jelly-derived MSCs in enhancing peripheral nerve regeneration.
Mechanisms of Wharton's Jelly-derived MSCs in enhancing peripheral nerve regeneration.
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DOI：
10.1038/s41598-023-48495-6
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发表时间：
2023-12-01
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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作者：
Warton’s骨髓间充质干细胞（WJ-MSCs）在促进无细胞神经移植物（ANG）的神经再生中起着关键作用;然而，WJ-MSCs相关神经再生的机制仍不清楚。本研究通过检测WJ-MSCs的免疫调节和旁分泌作用以及分化潜能，探讨了WJ-MSCs如何促进周围神经再生。为此，从脐带中分离WJ-MSCs，并将ANG（对照）或负载WJ-MSCs的ANG（WJ-MSCs组）移植到损伤动物模型中。术后16周通过踝关节角度和强直力测量评估功能恢复。移植后3、7和14天的组织活检用于通过定量实时聚合酶链反应（RT-qPCR）和免疫荧光染色分析巨噬细胞标志物和白细胞介素（IL）水平、旁分泌效应和MSC分化潜能。与对照组相比，WJ-MSCs组在4周时踝关节角度显著增加，在16周时等长强直力显著增加，在7 d和14 d时CD 206和IL 10的表达显著增加。在第14天观察到神经营养因子和血管生长因子水平增加。WJ-MSCs组显示出较高的S100β表达水平;然而，人细胞核的共染色较弱。这项研究表明，WJ-MSCs的免疫调节和旁分泌作用有助于周围神经再生超过其分化潜力。
Warton’s jelly-derived Mesenchymal stem cells (WJ-MSCs) play key roles in improving nerve regeneration in acellular nerve grafts (ANGs); however, the mechanism of WJ-MSCs-related nerve regeneration remains unclear. This study investigated how WJ-MSCs contribute to peripheral nerve regeneration by examining immunomodulatory and paracrine effects, and differentiation potential. To this end, WJ-MSCs were isolated from umbilical cords, and ANGs (control) or WJ-MSCs-loaded ANGs (WJ-MSCs group) were transplanted in injury animal model. Functional recovery was evaluated by ankle angle and tetanic force measurements up to 16 weeks post-surgery. Tissue biopsies at 3, 7, and 14 days post-transplantation were used to analyze macrophage markers and interleukin (IL) levels, paracrine effects, and MSC differentiation potential by quantitative real-time polymerase chain reaction (RT-qPCR) and immunofluorescence staining. The WJ-MSCs group showed significantly higher ankle angle at 4 weeks and higher isometric tetanic force at 16 weeks, and increased expression of CD206 and IL10 at 7 or 14 days than the control group. Increased levels of neurotrophic and vascular growth factors were observed at 14 days. The WJ-MSCs group showed higher expression levels of S100β; however, the co-staining of human nuclei was faint. This study demonstrates that WJ-MSCs' immunomodulation and paracrine actions contribute to peripheral nerve regeneration more than their differentiation potential.
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影响因子：
9
作者：
通讯作者：
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神经生长因子激活雪旺细胞中的自噬，增强髓鞘碎片清除并加速神经再生
影响因子：
12.4
作者：
Li, Rui;Li, Duohui;Xiao, Jian
通讯作者：
Xiao, Jian
影响因子：
6
作者：
Hopf A;Schaefer DJ;Kalbermatten DF;Guzman R;Madduri S
通讯作者：
Madduri S
影响因子：
5.6
作者：
Jung N;Park S;Choi Y;Park JW;Hong YB;Park HH;Yu Y;Kwak G;Kim HS;Ryu KH;Kim JK;Jo I;Choi BO;Jung SC
通讯作者：
Jung SC
影响因子：
1.3
作者：
Iyer SS;Cheng G
通讯作者：
Cheng G