Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration.
Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration.
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DOI：
10.3390/ijms17111867
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发表时间：
2016-11-09
影响因子：
5.6
通讯作者：
Jung SC
中科院分区：
文献类型：
--
作者：
Jung N;Park S;Choi Y;Park JW;Hong YB;Park HH;Yu Y;Kwak G;Kim HS;Ryu KH;Kim JK;Jo I;Choi BO;Jung SC
雪旺细胞（SCs）产生神经营养因子和粘附分子，在轴突再生过程中起到结构支持和引导作用；因此，它们可能是修复受损神经组织的关键靶点。自体SC移植在治疗神经损伤和供体部位病变方面已显示出良好的临床效果，但细胞产生不足一直被认为是一个主要问题。在这里，我们将扁桃体来源的间充质干细胞（T-MSCs）分化为sc样细胞（T-MSCs -SCs），以评估T-MSCs作为sc的替代品。通过实时荧光定量PCR检测SC标志物CAD19、GFAP、MBP、NGFR、S100B和KROX20在完全分化阶段的表达上调以及CAD19和MBP的下调。此外，我们发现分化的SCs与小鼠背根神经节神经元共培养时轴突有髓鞘形成。将T-MSC-SCs应用于坐骨神经损伤小鼠模型，可显著改善步态，促进受损神经的再生。因此，移植人T-MSCs可能适合于辅助周围神经再生。
Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been performed and has shown promising clinical results for treating nerve injuries and donor site morbidity, and insufficient production of the cells have been considered as a major issue. Here, we performed differentiation of tonsil-derived mesenchymal stem cells (T-MSCs) into SC-like cells (T-MSC-SCs), to evaluate T-MSC-SCs as an alternative to SCs. Using SC markers such as CAD19, GFAP, MBP, NGFR, S100B, and KROX20 during quantitative real-time PCR we detected the upregulation of NGFR, S100B, and KROX20 and the downregulation of CAD19 and MBP at the fully differentiated stage. Furthermore, we found myelination of axons when differentiated SCs were cocultured with mouse dorsal root ganglion neurons. The application of T-MSC-SCs to a mouse model of sciatic nerve injury produced marked improvements in gait and promoted regeneration of damaged nerves. Thus, the transplantation of human T-MSCs might be suitable for assisting in peripheral nerve regeneration.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/j.biocel.2004.02.023
发表时间：
2004-10-01
影响因子：
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作者：
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通讯作者：
Jessen, KR
影响因子：
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作者：
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