Long-distance endosome trafficking drives fungal effector production during plant infection.
Long-distance endosome trafficking drives fungal effector production during plant infection.
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DOI：
10.1038/ncomms6097
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发表时间：
2014-10-06
影响因子：
16.6
通讯作者：
Steinberg, Gero
中科院分区：
文献类型：
--
作者：
Bielska, Ewa;Higuchi, Yujiro;Schuster, Martin;Steinberg, Natascha;Kilaru, Sreedhar;Talbot, Nicholas J.;Steinberg, Gero
为了引发植物病害，病原真菌可将效应蛋白分泌到植物细胞中，以抑制植物免疫并促进真菌侵染。大多数真菌病原体利用非常长的丝状细胞（称为菌丝）侵染植物，这些菌丝从其顶端将效应物分泌到宿主组织中。真菌在侵染过程中如何进行长距离细胞信号传导以调控效应物的产生尚不清楚。在此我们表明，在病原真菌玉米黑粉菌侵染植物期间，早期内体的长距离逆向运动对于触发效应物编码基因的转录是必需的。我们证明，依赖马达的早期内体逆向运动对于宿主细胞入侵过程中效应物产生和分泌的调控是必需的。我们进一步表明，逆向信号传导涉及在早期内体上移动并参与效应物产生控制的促分裂原活化激酶Crk1。因此，真菌病原体进行长距离信号传导以精心策划宿主入侵。
目前尚不清楚在植物组织入侵过程中，非常长的真菌细胞（菌丝）的细胞核如何从菌丝顶端接收信息。在此，作者表明，从菌丝顶端到细胞核的早期内体逆向运动是这一信号传导过程所必需的。
To cause plant disease, pathogenic fungi can secrete effector proteins into plant cells to suppress plant immunity and facilitate fungal infection. Most fungal pathogens infect plants using very long strand-like cells, called hyphae, that secrete effectors from their tips into host tissue. How fungi undergo long-distance cell signalling to regulate effector production during infection is not known. Here we show that long-distance retrograde motility of early endosomes (EEs) is necessary to trigger transcription of effector-encoding genes during plant infection by the pathogenic fungus Ustilago maydis. We demonstrate that motor-dependent retrograde EE motility is necessary for regulation of effector production and secretion during host cell invasion. We further show that retrograde signalling involves the mitogen-activated kinase Crk1 that travels on EEs and participates in control of effector production. Fungal pathogens therefore undergo long-range signalling to orchestrate host invasion. It is unclear how the nuclei of very long fungal cells (hyphae) receive information from the hyphal tips during the invasion of plant tissues. Here, the authors show that retrograde movement of early endosomes, from the hyphal tip to the nucleus, is required for this signalling process.
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影响因子：
7.4
作者：
APOSTOL, I;HEINSTEIN, PF;LOW, PS
通讯作者：
LOW, PS
影响因子：
4.5
作者：
Abenza, Juan F.;Pantazopoulou, Areti;Penalva, Miguel A.
通讯作者：
Penalva, Miguel A.
影响因子：
3.6
作者：
Garrido, E;Pérez-Martín, J
通讯作者：
Pérez-Martín, J
影响因子：
--
作者：
Bononi A;Agnoletto C;De Marchi E;Marchi S;Patergnani S;Bonora M;Giorgi C;Missiroli S;Poletti F;Rimessi A;Pinton P
通讯作者：
Pinton P
影响因子：
4.8
作者：
Chen, Ye-Guang;Wang, Zhi;Lu, Zhongxian
通讯作者：
Lu, Zhongxian