The Arabidopsis DREAM complex antagonizes WDR5A to modulate histone H3K4me2/3 deposition for a subset of genome repression.
The Arabidopsis DREAM complex antagonizes WDR5A to modulate histone H3K4me2/3 deposition for a subset of genome repression.
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拟南芥 DREAM 复合物拮抗 WDR5A 以调节组蛋白 H3K4me2/3 沉积，从而实现基因组抑制的子集
DOI：
10.1073/pnas.2206075119
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发表时间：
2022-07-05
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
--
作者：
Transcriptional repressors such as the DREAM (dimerization partner, RB-like, E2F and multivulval class B)-related complexes are master regulators of the cell cycle, but much remains unknown about how binding of DREAM complexes controls gene expression. In this study, through a forward genetic screen we identified a plant-specific component of DREAM complex BTE1 (barrier of transcription elongation 1) in Arabidopsis. Our data showed that the interaction of BTE1 in the plant DREAM complex with Complex Proteins Associated with Set1 (COMPASS)-like complex component WDR5A contributes to the conserved DREAM complex-mediated transcription repression. H3K4me3 modification conferred by the COMPASS-like complex and methyltransferases in Arabidopsis is important for gene-specific RNA polymerase II (Pol II) transcription. We thus identified BTE1 as a key adaptor directly linking the promoter recognition and repression of Pol II elongation via the modulation of H3K4me3. The master transcriptional repressor DREAM (dimerization partner, RB-like, E2F and multivulval class B) complex regulates the cell cycle in eukaryotes, but much remains unknown about how it transmits repressive signals on chromatin to the primary transcriptional machinery (e.g., RNA polymerase II [Pol II]). Through a forward genetic screen, we identified BTE1 (barrier of transcription elongation 1), a plant-specific component of the DREAM complex. The subsequent characterization demonstrated that DREAM complex containing BTE1 antagonizes the activity of Complex Proteins Associated with Set1 (COMPASS)-like complex to repress H3K4me3 occupancy and inhibits Pol II elongation at DREAM target genes. We showed that BTE1 is recruited to chromatin at the promoter-proximal regions of target genes by E2F transcription factors. DREAM target genes exhibit characteristic enrichment of H2A.Z and H3K4me2 modification on chromatin. We further showed that BTE1 directly interacts with WDR5A, a core component of COMPASS-like complex, repressing WDR5A chromatin binding and the elongation of transcription on DREAM target genes. H3K4me3 is known to correlate with the Pol II transcription activation and promotes efficient elongation. Thus, our study illustrates a transcriptional repression mechanism by which the DREAM complex dampens H3K4me3 deposition at a set of genes through its interaction with WDR5A.
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影响因子：
10.5
作者：
Latorre I;Chesney MA;Garrigues JM;Stempor P;Appert A;Francesconi M;Strome S;Ahringer J
通讯作者：
Ahringer J
PCSD：植物染色质状态数据库
影响因子：
14.9
作者：
Liu Y;Tian T;Zhang K;You Q;Yan H;Zhao N;Yi X;Xu W;Su Z
通讯作者：
Su Z
拟南芥 ARGONAUTE 1 结合染色质促进基因转录以响应激素和应激
影响因子：
11.8
作者：
Liu, Chang;Xin, Ying;Qi, Yijun
通讯作者：
Qi, Yijun
影响因子：
4.5
作者：
Ding Y;Ndamukong I;Xu Z;Lapko H;Fromm M;Avramova Z
通讯作者：
Avramova Z
影响因子：
11.4
作者：
Derkacheva, Maria;Steinbach, Yvonne;Hennig, Lars
通讯作者：
Hennig, Lars