Visually Driven Neuropil Activity and Information Encoding in Mouse Primary Visual Cortex.
Visually Driven Neuropil Activity and Information Encoding in Mouse Primary Visual Cortex.
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DOI：
10.3389/fncir.2017.00050
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发表时间：
2017
影响因子：
3.5
通讯作者：
Smirnakis SM
中科院分区：
文献类型：
--
作者：
Lee S;Meyer JF;Park J;Smirnakis SM
通过钙成像记录的皮质神经元调节反映了来自大量神经元的轴-树突过程和突触隔室的大聚集体的活性。这种活动的组织影响神经元放电，但还没有很好地理解。在这里，我们使用在体双光子成像与俄勒冈州绿色Bapta（OGB）和GCaMP 6研究神经元视觉反应的移动光栅在2/3层的小鼠V1区。我们发现，神经元的反应是强烈的调制和更可靠的比邻近的躯体活动。此外，神经元活动中的刺激独立调节，即，噪声相关性在皮层表面高度一致，距离至少为200 μm。成对的神经纤毛-神经纤毛-斑块噪声相关强度远高于细胞-细胞噪声相关强度，并且强烈依赖于大脑状态，相对于轻度麻醉，安静清醒时降低。神经元噪声相关强度随距离的增加而逐渐降低，在200 μm处下降了约11%。这比细胞间噪声相关性的曲线相对较慢，后者在200 μm处下降了约23%。有趣的是，尽管小鼠V1神经元的方向和方向编码的“盐和胡椒”组织，神经元斑块的群体，即使是中等大小（半径约100 μm），也显示出区分垂直移动光栅的高准确性。这与相应细胞群的准确度相当。神经元活动的动态、刺激依赖性的性质进一步强调了在当代成像研究中将神经元活动与细胞索马活动仔细分离的必要性。
Cortical neuropil modulations recorded by calcium imaging reflect the activity of large aggregates of axo-dendritic processes and synaptic compartments from a large number of neurons. The organization of this activity impacts neuronal firing but is not well understood. Here we used in vivo 2-photon imaging with Oregon Green Bapta (OGB) and GCaMP6s to study neuropil visual responses to moving gratings in layer 2/3 of mouse area V1. We found neuropil responses to be strongly modulated and more reliable than neighboring somatic activity. Furthermore, stimulus independent modulations in neuropil activity, i.e., noise correlations, were highly coherent across the cortical surface, up to distances of at least 200 μm. Pairwise neuropil-to-neuropil-patch noise correlation strength was much higher than cell-to-cell noise correlation strength and depended strongly on brain state, decreasing in quiet wakefulness relative to light anesthesia. The profile of neuropil noise correlation strength decreased gently with distance, dropping by ~11% at a distance of 200 μm. This was comparatively slower than the profile of cell-to-cell noise correlations, which dropped by ~23% at 200 μm. Interestingly, in spite of the “salt & pepper” organization of orientation and direction encoding across mouse V1 neurons, populations of neuropil patches, even of moderately large size (radius ~100 μm), showed high accuracy for discriminating perpendicularly moving gratings. This was commensurate to the accuracy of corresponding cell populations. The dynamic, stimulus dependent, nature of neuropil activity further underscores the need to carefully separate neuropil from cell soma activity in contemporary imaging studies.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
16.2
作者：
Ecker AS;Berens P;Cotton RJ;Subramaniyan M;Denfield GH;Cadwell CR;Smirnakis SM;Bethge M;Tolias AS
通讯作者：
Tolias AS
影响因子：
5.8
作者：
Kim, Hyunsoo;Park, Haesun
通讯作者：
Park, Haesun
影响因子：
16.2
作者：
Niell CM;Stryker MP
通讯作者：
Stryker MP
影响因子：
48
作者：
Nimmerjahn, A;Kirchhoff, F;Helmchen, F
通讯作者：
Helmchen, F