Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol.
Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol.
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DOI：
10.1186/2191-0855-3-20
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发表时间：
2013-04-02
期刊：
影响因子：
3.7
通讯作者：
Habe H
中科院分区：
文献类型：
--
作者：
Sato S;Morita N;Kitamoto D;Yakushi T;Matsushita K;Habe H
为了提高生物柴油生产中含甲醇原料甘油的增值利用，研究了添加甲醇对葡萄杆菌生产甘油三酯（GA）的影响。我们首先以生甘油为进料液，用弗拉特利葡萄糖杆菌NBRC103465进行分批补料发酵。当原料甘油中含有甲醇时，随着二羟基丙酮（DHA）生成的增加，GA产率降低。本实验的结果和以原料甘油为模型的合成溶液的对比实验表明，甲醇的存在导致发酵过程中产生的两种甘油衍生物GA和DHA的浓度发生变化。其他葡萄球菌在1% （v/v）甲醇的存在下也会降低GA的产量。此外，从氧化葡萄糖杆菌中纯化的膜结合乙醇脱氢酶（mADH）是GA生产的关键酶，随着甲醇浓度的增加，脱氢酶对甘油的活性降低。这些结果强烈表明，葡萄糖杆菌产生GA的减少是由于甲醇诱导的madh介导的甘油氧化的抑制。
To enhance the value-added use of methanol-containing raw glycerol derived from biodiesel fuel production, the effect of methanol supplementation on glyceric acid (GA) production by Gluconobacter spp. was investigated. We first conducted fed-batch fermentation with Gluconobacter frateurii NBRC103465 using raw glycerol as a feeding solution. GA productivity decreased with increasing dihydroxyacetone (DHA) formation when the raw glycerol contained methanol. The results of this experiment and comparative experiments using a synthetic solution modeled after the raw glycerol indicate that the presence of methanol caused a change in the concentrations of GA and DHA, two glycerol derivatives produced during fermentation. Other Gluconobacter spp. also decreased GA production in the presence of 1% (v/v) methanol. In addition, purified membrane-bound alcohol dehydrogenase (mADH) from Gluconobacter oxydans, which is a key enzyme in GA production, showed a decrease in dehydrogenase activity toward glycerol as the methanol concentration increased. These results strongly suggest that the observed decrease in GA production by Gluconobacter spp. resulted from the methanol-induced inhibition of mADH-mediated glycerol oxidation.
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DOI：
10.1080/00021369.1978.10863306
发表时间：
1978-01-01
期刊：
AGRICULTURAL AND BIOLOGICAL CHEMISTRY
影响因子：
--
作者：
ADACHI, O;TAYAMA, K;AMEYAMA, M
通讯作者：
AMEYAMA, M
影响因子：
3.4
作者：
Asad-ur-Rehman;Saman, Wijesekara R. G.;Matsumura, Masatoshi
通讯作者：
Matsumura, Masatoshi
影响因子：
11.4
作者：
Hu, Zhong-Ce;Zheng, Yu-Guo;Shen, Yin-Chu
通讯作者：
Shen, Yin-Chu
影响因子：
1.6
作者：
Soemphol, Wichai;Adachi, Osao;Toyama, Hirohide
通讯作者：
Toyama, Hirohide
影响因子：
2.6
作者：
CLARET, C;BORIES, A;SOUCAILLE, P
通讯作者：
SOUCAILLE, P