Global analyses of mRNA translational control during early Drosophila embryogenesis.
Global analyses of mRNA translational control during early Drosophila embryogenesis.
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DOI：
10.1186/gb-2007-8-4-r63
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Rubin GM
中科院分区：
文献类型：
--
作者：
Qin X;Ahn S;Speed TP;Rubin GM
产卵后前 10 小时内超过 15,000 个转录本的多核糖体谱已确定。在许多动物中，生命的最初几个小时几乎没有转录，并且这些早期阶段的发育调节依赖于母体细胞质而不是合子核。翻译控制对于早期果蝇胚胎发生至关重要，并且主要在基因水平上发挥作用。为了了解果蝇早期胚胎发育过程中的转录后调控，我们使用蔗糖多聚体梯度分析和基因芯片分析来说明单个 mRNA 的翻译谱。我们在产卵后的前十个小时内测定了超过 10,000 个转录本的核糖体密度和核糖体占用率。我们在全基因组基础上报告了果蝇早期胚胎发生过程中翻译水平上基因调控的程度和一般性质。翻译概况的多样性表明调节转录本特异性翻译的多种机制。聚类分析表明，参与某些生物过程的基因在某些发育阶段在翻译水平上受到共同调控。
The polysomal profiles of over 15,000 transcripts during the first ten hours after egg laying have been determined. In many animals, the first few hours of life proceed with little or no transcription, and developmental regulation at these early stages is dependent on maternal cytoplasm rather than the zygotic nucleus. Translational control is critical for early Drosophila embryogenesis and is exerted mainly at the gene level. To understand post-transcriptional regulation during Drosophila early embryonic development, we used sucrose polysomal gradient analyses and GeneChip analysis to illustrate the translation profile of individual mRNAs. We determined ribosomal density and ribosomal occupancy of over 10,000 transcripts during the first ten hours after egg laying. We report the extent and general nature of gene regulation at the translational level during early Drosophila embryogenesis on a genome-wide basis. The diversity of the translation profiles indicates multiple mechanisms modulating transcript-specific translation. Cluster analyses suggest that the genes involved in some biological processes are co-regulated at the translational level at certain developmental stages.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
64.5
作者：
EDGAR, BA;SCHUBIGER, G
通讯作者：
SCHUBIGER, G
影响因子：
4.8
作者：
Qin, XL;Sarnow, P
通讯作者：
Sarnow, P
影响因子：
64.8
作者：
MACDONALD, PM;STRUHL, G
通讯作者：
STRUHL, G
影响因子：
64.5
作者：
Groisman, I;Jung, MY;Richter, JD
通讯作者：
Richter, JD