Up-regulation of miR-203 expression induces endothelial inflammatory response: Potential role in preeclampsia.
Up-regulation of miR-203 expression induces endothelial inflammatory response: Potential role in preeclampsia.
复制标题
miR-203 表达上调诱导内皮炎症反应：在先兆子痫中的潜在作用。
DOI：
10.1111/aji.12589
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Groome LJ
中科院分区：
文献类型：
--
作者：
Wang Y;Dong Q;Gu Y;Groome LJ
确定miR-203是否介导子痫前期的内皮炎症反应。原位杂交检测母体血管miR-203的表达。免疫染色法检测细胞因子信号传导抑制因子-3 （SOCS-3）和ICAM的表达。使用正常和子痫前期孕妇的皮下脂肪组织切片。通过测量转染MiR-203前体pre-miR-203的内皮细胞（EC）中IL-6、IL-8、ICAM和VCAM的表达和产生，以及中性粒细胞粘附，评估MiR-203诱导的炎症反应。测定SOCS3的表达。子痫前期母体血管内皮中miR-203和ICAM表达上调，SOCS-3表达下调。过表达miR-203导致细胞中SOCS-3表达下调，IL-6、IL-8、ICAM和VCAM的产生增加，中性粒细胞粘附增加。由于miR-203是一种炎症性microRNA，在ECs中miR-203的产生/表达增加可能会降低子痫前期内皮细胞的抗炎活性，增加内皮细胞的炎症反应。
To determine if miR-203 mediates endothelial inflammatory response in preeclampsia. Maternal vessel miR-203 expression was assessed by in situ hybridization. Suppressor of cytokine signaling-3 (SOCS-3) and ICAM expression was determined by immunostaining. Subcutaneous fat tissue sections from normal and preeclamptic pregnant women were used. MiR-203 induced inflammatory response was evaluated by measurements of IL-6, IL-8, ICAM, and VCAM expression and production, and neutrophil adhesion in endothelial cells (EC) transfected with miR-203 precursor, pre-miR-203. SOCS3 expression was also determined. Up-regulation of miR-203 and ICAM expression and down-regulation of SOCS-3 expression was demonstrated in maternal vessel endothelium in preeclampsia. Over-expression of miR-203 resulted in down-regulation of SOCS-3 expression and increases in production of IL-6, IL-8, ICAM and VCAM, and neutrophil adhesion in ECs. As miR-203 is an inflammatory microRNA, increased miR-203 production/ expression in ECs could diminish anti-inflammatory activity and increase endothelial inflammatory response in preeclampsia.
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DOI：
10.4049/jimmunol.0902369
发表时间：
2010-01-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Suárez Y;Wang C;Manes TD;Pober JS
通讯作者：
Pober JS
影响因子：
32.4
作者：
O'Connell RM;Kahn D;Gibson WS;Round JL;Scholz RL;Chaudhuri AA;Kahn ME;Rao DS;Baltimore D
通讯作者：
Baltimore D
DOI：
10.1152/ajpendo.00660.2012
发表时间：
2013-04-01
影响因子：
5.1
作者：
Gu, Yang;Sun, Jingxia;Wang, Yuping
通讯作者：
Wang, Yuping
影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通讯作者：
MINICK, CR
影响因子：
3.1
作者：
Moffatt, Catherine E.;Lamont, Richard J.
通讯作者：
Lamont, Richard J.