Acute dietary zinc deficiency before conception compromises oocyte epigenetic programming and disrupts embryonic development.
Acute dietary zinc deficiency before conception compromises oocyte epigenetic programming and disrupts embryonic development.
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DOI：
10.1016/j.ydbio.2013.01.015
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发表时间：
2013-04-01
影响因子：
2.7
通讯作者：
Diaz, F. J.
中科院分区：
文献类型：
--
作者：
Tian, X.;Diaz, F. J.
最近的研究表明，锌是调节减数分裂细胞周期和排卵的重要因素。然而，锌在促进卵母细胞质量和发育潜力方面的作用尚不清楚。使用急性饮食锌缺乏的体内模型，我们表明，在排卵（孕前）前3-5天喂养缺锌饮食（ZDD）显着破坏卵母细胞染色质甲基化和植入前发育。缺锌卵母细胞组蛋白H3 K4三甲基化和DNA甲基化水平显著降低。此外，重复元件（Iap、Line 1、Sineb 1、Sineb 2）的转录丰度增加3-20倍，但Gdf 9、Zp 3和Figla mRNA的转录丰度降低。在接受3天和5天ZDD的动物中，分别只有53%和8%的成熟卵子在IVF后达到2-细胞阶段，而体内5天ZDD将2-细胞的比例降低至49%。体外培养的体内受精2-细胞胚胎形成的囊胚（38%）少于对照胚胎（74%）。同样，在妊娠第3.5天，从缺锌动物的生殖道中收集到的囊胚和扩张囊胚较少。这可能是由于ZDD囊胚中Igf 2和H19 mRNA的减少。在IVM期间补充甲基供体（SAM）恢复了组蛋白H3 K4 me 3，并使缺锌动物卵母细胞的IVF成功率从17%增加到43%。因此，卵母细胞发育的终末期对膳食锌供应的扰动极其敏感。
Recent findings show that zinc is an important factor necessary for regulating the meiotic cell cycle and ovulation. However, the role of zinc in promoting oocyte quality and developmental potential is not known. Using an in vivo model of acute dietary zinc deficiency, we show that feeding a zinc deficient diet (ZDD) for 3–5 days before ovulation (preconception) dramatically disrupts oocyte chromatin methylation and preimplantation development. There was a dramatic decrease in histone H3K4 trimethylation and global DNA methylation in zinc deficient oocytes. Moreover, there was a 3–20 fold increase in transcript abundance of repetitive elements (Iap, Line1, Sineb1, Sineb2), but a decrease in Gdf9, Zp3 and Figla mRNA. Only 53% and 8% of mature eggs reached the 2-cell stage after IVF in animals receiving a 3 and 5 day ZDD, respectively, while a 5 day ZDD in vivo reduced the proportion of 2-cells to 49%. In vivo fertilized 2-cell embryos cultured in vitro formed fewer (38%) blastocysts compared to control embryos (74%). Likewise, fewer blastocyst and expanded blastocyst were collected from the reproductive tract of zinc deficient animals on day 3.5 of pregnancy. This could be due to a decrease in Igf2 and H19 mRNA in ZDD blastocyst. Supplementation with a methyl donor (SAM) during IVM restored histone H3K4me3 and doubled the IVF success rate from 17% to 43% in oocytes from zinc deficient animals. Thus, the terminal period of oocyte development is extremely sensitive to perturbation in dietary zinc availability.
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影响因子：
6.1
作者：
Berker, Buelent;Kaya, Cemil;Satiroglu, Hakan
通讯作者：
Satiroglu, Hakan
影响因子：
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作者：
Breksa, AP;Garrow, TA
通讯作者：
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在小鼠表观遗传敏感等位基因上DNA甲基化的动态重编程。
影响因子：
4.5
作者：
Blewitt ME;Vickaryous NK;Paldi A;Koseki H;Whitelaw E
通讯作者：
Whitelaw E
影响因子：
3.6
作者：
Bernhardt, Miranda L.;Kong, Betty Y.;Woodruff, Teresa K.
通讯作者：
Woodruff, Teresa K.