Nitrogen deficiency in barley (Hordeum vulgare) seedlings induces molecular and metabolic adjustments that trigger aphid resistance.
Nitrogen deficiency in barley (Hordeum vulgare) seedlings induces molecular and metabolic adjustments that trigger aphid resistance.
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DOI：
10.1093/jxb/erv276
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发表时间：
2015-06
影响因子：
6.9
通讯作者：
Hancock RD
中科院分区：
文献类型：
--
作者：
Comadira G;Rasool B;Karpinska B;Morris J;Verrall SR;Hedley PE;Foyer CH;Hancock RD
氮缺乏诱导大麦叶片广泛的代谢调节，主要通过糖信号和与生物胁迫途径重叠的受体样激酶级联来诱导抗虫性。使用合成肥料造成的农业一氧化二氮(N2O)污染是人为温室气体排放的重要组成部分，为减少使用氮肥(N)提供了理由。氮素限制会导致广泛的系统再平衡，从而重塑新陈代谢和防御过程。为了分析支持这些反应的调控机制，大麦幼苗在缺氮条件下生长7d，直到净光合作用比全氮对照低50%。虽然枝条生长减少，但没有证据表明氧化胁迫的诱导，尽管含N的抗氧化剂的总浓度较低。缺氮大麦叶片富含氨基酸、糖和三羧酸循环中间体。与富氮叶片相比，桃蚜1日龄若虫转移到缺氮大麦叶片后未能成虫。在N缺乏的叶片中，编码细胞、糖和营养信号、蛋白质降解和次生代谢的转录本过度表达，而与激素代谢相关的转录本在两种营养条件下相似，但编码生长素代谢和反应的蛋白质的mRNAs除外。N限制大麦叶片转录组与受棉铃虫侵染的拟南芥叶片的转录组有显著的相似性。这些发现不仅突出了生物和非生物胁迫信号级联之间的显著相似之处，而且还确定了提高抗虫性的潜在目标，这对可持续农业的发展具有重要意义。
Nitrogen deficiency induces extensive metabolic adjustment in barley leaves mediated largely by sugar signalling and receptor-like kinase cascades that overlap with biotic stress pathways to induce aphid resistance. Agricultural nitrous oxide (N2O) pollution resulting from the use of synthetic fertilizers represents a significant contribution to anthropogenic greenhouse gas emissions, providing a rationale for reduced use of nitrogen (N) fertilizers. Nitrogen limitation results in extensive systems rebalancing that remodels metabolism and defence processes. To analyse the regulation underpinning these responses, barley (Horedeum vulgare) seedlings were grown for 7 d under N-deficient conditions until net photosynthesis was 50% lower than in N-replete controls. Although shoot growth was decreased there was no evidence for the induction of oxidative stress despite lower total concentrations of N-containing antioxidants. Nitrogen-deficient barley leaves were rich in amino acids, sugars and tricarboxylic acid cycle intermediates. In contrast to N-replete leaves one-day-old nymphs of the green peach aphid (Myzus persicae) failed to reach adulthood when transferred to N-deficient barley leaves. Transcripts encoding cell, sugar and nutrient signalling, protein degradation and secondary metabolism were over-represented in N-deficient leaves while those associated with hormone metabolism were similar under both nutrient regimes with the exception of mRNAs encoding proteins involved in auxin metabolism and responses. Significant similarities were observed between the N-limited barley leaf transcriptome and that of aphid-infested Arabidopsis leaves. These findings not only highlight significant similarities between biotic and abiotic stress signalling cascades but also identify potential targets for increasing aphid resistance with implications for the development of sustainable agriculture.
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影响因子：
6.9
作者：
Amiour, Nardjis;Imbaud, Sandrine;Hirel, Bertrand
通讯作者：
Hirel, Bertrand
影响因子：
9.4
作者：
Jukanti, Aravind K.;Heidlebaugh, Nancy M.;Fischer, Andreas M.
通讯作者：
Fischer, Andreas M.
影响因子：
6.9
作者：
Foyer, CH;Parry, M;Noctor, G
通讯作者：
Noctor, G
DOI：
10.1073/pnas.232703799
发表时间：
2003-01-07
影响因子：
11.1
作者：
Balmer, Y;Koller, A;Buchanan, BB
通讯作者：
Buchanan, BB
DOI：
10.1073/pnas.0914216107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Burney, Jennifer A.;Davis, Steven J.;Lobell, David B.
通讯作者：
Lobell, David B.