QTL mapping reveals candidate genes for main agronomic traits in Luffa based on a high-resolution genetic map.
QTL mapping reveals candidate genes for main agronomic traits in Luffa based on a high-resolution genetic map.
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基于高分辨率遗传图谱的QTL定位揭示了丝瓜主要农艺性状的候选基因
DOI：
10.3389/fpls.2022.1069618
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
丝瓜是葫芦科重要的药食蔬菜作物。针刺丝瓜和白根丝瓜两个驯化丝瓜品种间存在着较强的杂种优势效应和显著的互补特性。为了探究其重要农艺性状的遗传基础，我们利用丝瓜S1174与丝瓜P93075杂交的110个品系的BC1群体构建了首个种间高密度遗传连锁图谱。该地图总面积为2246.74 cM，相邻标记之间的平均距离为0.48 cM。随后，对25个重要农艺性状进行了大规模田间定量性状位点定位，检测到分布在11条染色体上的40个显著遗传位点。值得注意的是，位于9号染色体上最小距离为23 kb的重要QTL （qID2）被确定为节间直径的原因，并解释了11%的表型变异。Lac09g006860 （LacCRWN3）编码一种参与细胞核形态控制的核层蛋白，是qID2中唯一的编码基因。序列比对显示，LacCRWN3亲本等位基因启动子序列完全不同，但外显子存在非同义单核苷酸多态性（snp），且该基因在粗茎P93075中的表达量显著高于细茎S1174。通过对183个自交系群体的自然变异分析，发现LacCRWN3主要有两种单倍型：P93075-like和S1174-like，前者单倍型株系的节间直径明显大于后者。结果表明，LacCRWN3作为葫芦科唯一的CRWN3基因，是调控丝瓜节间直径的最有可能的候选基因。研究结果将有助于揭示丝瓜关键表型性状的分子机制，促进丝瓜的创客辅助育种。
Luffa is an important medicinal and edible vegetable crop of Cucurbitaceae. Strong heterosis effects and strikingly complementary characteristics were found between the two domesticated Luffa cultivars, Luffa acutangula and Luffa cylindrica. To explore the genetic basis underlying their important agronomic traits, we constructed the first interspecific high-density genetic linkage map using a BC1 population of 110 lines derived from a cross between S1174 (Luffa acutangula) and P93075 (Luffa cylindrica). The map spanned a total of 2246.74 cM with an average distance of 0.48 cM between adjacent markers. Thereafter, a large-scale field-based quantitative trait loci (QTLs) mapping was conducted for 25 important agronomic traits and 40 significant genetic loci distributed across 11 chromosomes were detected. Notably, a vital QTL (qID2) located on chromosome 9 with a minimum distance of 23 kb was identified to be responsible for the internode diameter and explained 11% of the phenotypic variation. Lac09g006860 (LacCRWN3), encoding a nuclear lamina protein involved in the control of nuclear morphology, was the only gene harbored in qID2. Sequence alignment showed completely different promoter sequences between the two parental alleles of LacCRWN3 except for some nonsynonymous single nucleotide polymorphisms (SNPs) in exons, and the expression level in thick-stem P93075 was distinctively higher than that in thin-stem S1174. According to the natural variation analysis of a population of 183 inbred lines, two main haplotypes were found for LacCRWN3: the P93075-like and S1174-like, with the former haplotype lines exhibiting significantly thicker internode diameters than those of the latter haplotype lines. It showed that LacCRWN3, as the only CRWN3 gene in Cucurbitaceae, was the most likely candidate gene regulating the internode diameter of Luffa. Our findings will be beneficial for deciphering the molecular mechanism of key phenotypic traits and promoting maker-assisted breeding in Luffa.
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
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影响因子：
4.6
作者：
Jiang, Li;Yan, Shuangshuang;Zhang, Xiaolan
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影响因子：
64.8
作者：
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通讯作者：
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