Design and synthesis of a ratiometric photoacoustic imaging probe activated by selenol for visual monitoring of pathological progression of autoimmune hepatitis.
Design and synthesis of a ratiometric photoacoustic imaging probe activated by selenol for visual monitoring of pathological progression of autoimmune hepatitis.
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硒醇激活的比率光声成像探针的设计与合成，用于视觉监测自身免疫性肝炎的病理进展
DOI：
10.1039/d0sc06573k
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发表时间：
2021-02-19
期刊：
影响因子：
8.4
通讯作者：
Zhao S
中科院分区：
文献类型：
--
作者：
Zhang C;Qiu Z;Zhang L;Pang Q;Yang Z;Qin JK;Liang H;Zhao S
光声成像兼具光学成像的高对比度和超声成像的高空间分辨率，是一种可靠的生物医学成像技术。自身免疫性肝炎（Autoimmune hepatitis，AIH）是仅次于病毒性肝炎的第二大肝脏炎症性疾病，其发病机制尚不完全清楚，可能是由于缺乏有效的体内监测方法。在这项工作中，创新的硒激活的比率PA成像探针APSel的视觉监测AIH的病理进展的发展。硒代半胱氨酸（Sec，体内含硒物质的主要形式）等硒醇已被证明在炎症中具有有效的抗氧化作用。APSel与硒醇的反应导致PA光谱峰从860 nm蓝移到690 nm，这使得比率PA成像成为可能。APSel探针对Sec和其他硒醇显示出高灵敏度和选择性。然后，APSel探针用于细胞中硒醇的比率PA成像，并通过跟踪硒醇水平的变化来监测小鼠模型中AIH的发展。结果表明，硒醇水平与AIH的发生发展密切相关。作为硒醇响应PA成像探针的第一个例子，所提出的APSel为研究和诊断AIH疾病提供了新的工具和方法。
Photoacoustic (PA) imaging with both the high contrast of optical imaging and the high spatial resolution of ultrasound imaging has been regarded as a robust biomedical imaging technique. Autoimmune hepatitis (AIH) is the second largest liver inflammatory disease after viral hepatitis, but its pathogenesis is not fully understood probably due to the lack of an effective in vivo monitoring approach. In this work, an innovative selenol-activated ratiometric PA imaging probe APSel was developed for visual monitoring of pathological progress of AIH. Selenols including selenocysteine (Sec, the major form of Se-containing species in vivo) have been demonstrated to have an effective antioxidant role in inflammation. The reaction of APSel with selenol results in a blue shift of the PA spectrum peak from 860 nm to 690 nm, which enables the ratiometric PA imaging. The APSel probe displays high sensitivity and selectivity to Sec and other selenols. The APSel probe was then employed for ratiometric PA imaging of selenol in cells, and for monitoring the development of AIH in a murine model by tracking the changes of selenol level. The results revealed that the level of selenol was closely correlated with the development of AIH. The proposed APSel, as the first example of a selenol-responsive PA imaging probe, provides a new tool and approach to study and diagnose AIH diseases.
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影响因子：
18.3
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