Altered respiratory microbiota composition and functionality associated with asthma early in life.
Altered respiratory microbiota composition and functionality associated with asthma early in life.
复制标题
DOI：
10.1186/s12879-020-05427-3
复制
发表时间：
2020-09-22
影响因子：
3.7
通讯作者：
Hamid Q
中科院分区：
文献类型：
--
作者：
Al Bataineh MT;Hamoudi RA;Dash NR;Ramakrishnan RK;Almasalmeh MA;Sharif HA;Al-Hajjaj MS;Hamid Q
呼吸道的微生物群在维持呼吸系统健康方面具有重要作用。然而，对中东人群中哮喘患者的呼吸道微生物群知之甚少。这项研究调查了阿联酋受试者中与哮喘相关的呼吸道微生物群组成和功能。我们进行了16S rRNA和ITS2基因为基础的微生物分析的40个成人和儿童阿联酋个人平均年龄分别为52岁和7岁，有或无哮喘的痰液样本。我们报告了哮喘和非哮喘对照组中属于拟杆菌门、厚壁菌门、梭杆菌门和变形菌门的细菌差异。同样，真菌属于子囊菌门，担子菌门和其他未分类的真菌的差异。与哮喘控制测试相关的哮喘个体中的差异丰度测试显示埃塞俄比亚青霉和链格孢属的显著消耗，控制不佳的哮喘患者此外，数据表明马拉色菌属的显著扩增。和其他未分类的真菌在接受类固醇和白三烯受体拮抗剂的联合治疗的患者的气道中，与单独接受类固醇的患者相反。16S数据的功能分析显示，儿童哮喘和非哮喘对照之间存在显著差异，儿童哮喘患者显示氨基酸水平增加，（p值< 5.03 × 10− 7），碳水化合物（p值< 4.76 × 10− 7）和脂肪酸降解（p值< 6.65 × 10− 7）途径，而非哮喘对照组与氨基酸水平增加相关。（p值< 8.34 × 10− 7）、碳水化合物（p值< 3.65 × 10− 7）和脂肪酸（p值< 2.18 × 10− 6）生物合成途径与肠型组成一致。这些差异提供了对阿联酋人群呼吸道微生物群组成及其在生命早期哮喘发展中可能作用的深入了解。这项研究提供了重要的信息，可能最终导致筛选生物标志物的发展，以预测早期哮喘的发展和新的治疗方法。
The microbiota of the respiratory tract has an important role in maintaining respiratory health. However, little is known on the respiratory microbiota in asthmatic patients among Middle Eastern populations. This study investigated the respiratory microbiota composition and functionality associated with asthma in Emirati subjects. We performed 16S rRNA and ITS2-gene based microbial profiling of 40 expectorated sputum samples from adult and pediatric Emirati individuals averaging 52 and 7 years of age, respectively with or without asthma. We report bacterial difference belonging to Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria phyla between asthmatic and non-asthmatic controls. Similarly, fungal difference belonging to Ascomycota, Basidiomycota phyla and other unclassified fungi. Differential abundance testing among asthmatic individuals with relation to Asthma Control Test show a significant depletion of Penicillium aethiopicum and Alternaria spp., among poorly controlled asthmatics. Moreover, data suggest a significant expansion of Malassezia spp. and other unclassified fungi in the airways of those receiving steroids and leukotriene receptor antagonists’ combination therapy, in contrast to those receiving steroids alone. Functional profiling from 16S data showed marked differences between pediatric asthmatic and non-asthmatic controls, with pediatric asthmatic patients showing an increase in amino acid (p-value < 5.03 × 10− 7), carbohydrate (p-value < 4.76 × 10− 7), and fatty acid degradation (p-value < 6.65 × 10− 7) pathways, whereas non-asthmatic controls are associated with increase in amino acid (p-value < 8.34 × 10− 7), carbohydrate (p-value < 3.65 × 10− 7), and fatty acid (p-value < 2.18 × 10− 6) biosynthesis pathways in concordance with enterotype composition. These differences provide an insight into respiratory microbiota composition in Emirati population and its possible role in the development of asthma early in life. This study provides important information that may eventually lead to the development of screening biomarkers to predict early asthma development and novel therapeutic approaches.
登录
查看更多内容
影响因子：
14.2
作者：
Marri, Pradeep Reddy;Stern, Debra A.;Wright, Anne L.;Billheimer, Dean;Martinez, Fernando D.
通讯作者：
Martinez, Fernando D.
影响因子：
4
作者：
Mathieu E;Escribano-Vazquez U;Descamps D;Cherbuy C;Langella P;Riffault S;Remot A;Thomas M
通讯作者：
Thomas M
影响因子：
3.2
作者：
de Vries, Stefan P. W.;van Hijum, Sacha A. F. T.;Bootsma, Hester J.
通讯作者：
Bootsma, Hester J.
DOI：
10.1016/j.jaci.2010.10.048
发表时间：
2011-02
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Huang YJ;Nelson CE;Brodie EL;Desantis TZ;Baek MS;Liu J;Woyke T;Allgaier M;Bristow J;Wiener-Kronish JP;Sutherland ER;King TS;Icitovic N;Martin RJ;Calhoun WJ;Castro M;Denlinger LC;Dimango E;Kraft M;Peters SP;Wasserman SI;Wechsler ME;Boushey HA;Lynch SV;National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
影响因子：
158.5
作者：
Bisgaard, Hans;Hermansen, Mette Northman;Pipper, Christian Bressen
通讯作者：
Pipper, Christian Bressen